水性环氧树脂降解菌的驯化培养及筛选鉴定  

Domestication Culture and Screening Identification of WaterborneEpoxy Resin Degrading Bacteria

作　　者：李竺芯 樊迪 刘建宽 薛清月 于海航 阎松[1] LI Zhuxin;FAN Di;LIU Jiankuan;XUE Qingyue;YU Haihang;YAN Song(School of Environmental and Chemical Engineering,Dalian Jiaotong University,Dalian 116028,China)

机构地区：[1]大连交通大学环境与化学工程学院,辽宁大连116028

出　　处：《大连交通大学学报》2020年第2期90-93,共4页Journal of Dalian Jiaotong University

基　　金：国家自然科学基金资助项目(51408094);2018年国家级大学生创新创业训练计划资助项目。

摘　　要：从大连交通大学的池塘污水中分离出水性环氧树脂降解菌,经过驯化培养,从中纯化筛选出生长情况好的JDY(黄色)菌株和JDW(大白)菌株.通过革兰氏染色和分子生物学方法对JDY(黄色)菌株和JDW(大白)菌株进行了系统发育分析和种属鉴定.结果表明,JDW(大白)菌株属于假单胞菌属Pseudomonas sp.,JDY(黄色)菌株需要进一步鉴定.Several strains of waterborne epoxy resin degrading bacteria were isolated and purified from a pool in Dalian Jiaotong university.After domestication and cultivation,JDY and JDW waterborne epoxy resin degrading bacteria were isolated,which were more active.The JDY and JDW were determined by 16S rDNA alignment and phylogenesis analysis.The JDY is identified as Pseudomonas sp.,while the JDW is not clear.This study lays the foundation for the future study of the waterborne paint waste disposal.

关 键 词：水性环氧树脂降解菌 驯化 菌种鉴定 16Sr DNA 

分 类 号：X703[环境科学与工程—环境工程] X172