牛牌19大白菜杂交种种子纯度快速鉴定方法研究  

Studies on Rapid Identification on Purity of Chinese Cabbage‘Niupai No.19’Hybrid Seed

作　　者：刘栓桃 张志刚 王立华 王荣花 李巧云 赵智中 LIU Shuan-tao;ZHANG Zhi-gang;WANG Li-hua;WANG Rong-hua;LI Qiao-yun;ZHAO Zhi-zhong(Vegetable and Flower Institute of Shandong Academy of Agricultural Sciences,Shandong Branch of National Center for Vegetables Improvement,Shandong Key Laboratory for Biology of Greenhouse Vegetables,Huang-Huai-Hai Region Scientific Observation and Experimental Station of Vegetables,Ministry of Agriculture and Rural Affairs,Ji’nan 250100,Shandong,China)

机构地区：[1]山东省农业科学院蔬菜花卉研究所,国家蔬菜改良中心山东分中心,山东省设施蔬菜生物学重点实验室,农业农村部黄淮地区蔬菜科学观测实验站(山东),山东济南250100

出　　处：《中国蔬菜》2020年第3期28-32,共5页China Vegetables

基　　金：山东省农业重大应用技术创新项目(2018-2021);山东省农业良种工程项目(2019LZGC006);山东省重点研发计划项目(2014GSF21103)。

摘　　要：以牛牌19大白菜杂交种为试材,从种子预处理、DNA简便提取及质量检测、引物筛选等环节,构建了大白菜杂交种入库前分子标记快速鉴定技术体系,并与田间鉴定结果相比较。结果表明:该体系分子标记鉴定结果与田间种植法鉴定结果基本一致,杂交种纯度都是97%左右;以72 h萌发处理的单株幼苗为材料、采用两步法快速提取的DNA完全可以满足分子标记检测的需要。Taking Chinese cabbage‘Niupai No.19’hybrid seeds as test material,this paper constructed a rapid molecular marker testing technique system used before Chinese cabbage hybrid seeds being stored in genebank by every link,including seed pr-treating,DNA easy isolation and quality detection,primers screening,etc.and compared it with the result from fields identification.The results showed the identification results by molecular marker from the system were basically consistent with that by field planting.The purity of hybrids was about 97%.Taking the single plant seedlings treated for 72 hours to rapidly etract DNA by 2-step DNA isolation were sufficient for completely satisfying the needs of molecular marker detection.

关 键 词：大白菜 杂交种 入库前种子 分子标记 快速鉴定 

分 类 号：S634.1[农业科学—蔬菜学]