染色质转座酶可及性测序研究进展  

Advances in assay for transposase-accessible chromatin with high-throughput sequencing

作　　者：吴杰 全建平 叶勇 吴珍芳 杨杰 杨明 郑恩琴 Jie Wu;Jianping Quan;Yong Ye;ZhenFang Wu;Jie Yang;Ming Yang;Enqin Zheng(National Engineering Research Center for Breeding Swine Industry,College of Animal Science,South China Agricultural University,Guangzhou 510642,China;College of Animal Science and Technology,Zhongkai University of Agriculture and Engineering,Guangzhou 510225,China)

机构地区：[1]华南农业大学动物科学学院,国家生猪种业工程技术研究中心,广州510642 [2]仲恺农业工程学院,动物科技学院,广州510225

出　　处：《遗传》2020年第4期333-346,共14页Hereditas（Beijing)

基　　金：广东省“扬帆计划”引进创新创业团队项目(编号:2016YT03H062);广东省现代农业产业技术体系生猪创新团队项目(编号:2019KJ126);广东省自然科学基金项目(编号:2017A030313213)资助。

摘　　要：染色质转座酶可及性测序(assay for transposase-accessible chromatin with high-throughput sequencing,ATAC-seq)诞生于2013年,具有比脱氧核糖核酸酶I超敏感位点测序(deoxyribonuclease I hypersensitive site sequencing, DNase-seq)和微球菌核酸酶敏感位点测序(micrococcal nuclease sequencing, MNase-seq)更快速、灵敏、简便的优点,是目前分析全基因组范围染色质开放区域的热点技术。通过该技术能获得染色质开放区域的相关信息,从而映射出转录因子等调控蛋白的结合区域和核小体定位等信息,对于研究表观遗传分子机制具有重要意义。本文比较了5种获取染色质开放区域技术的优缺点,重点介绍了ATAC-seq的原理和主要流程,描述了利用ATAC-seq技术研究染色质开放区域的发展概况以及ATAC-seq的相关应用,期望对真核生物全基因组水平的染色质开放区域研究、顺式调控元件鉴定以及遗传调控网络的解析等提供借鉴。Assay for transposase accessible chromatin with high-throughput sequencing(ATAC-seq)was developed in 2013.It has the advantages of more convenient operation and higher efficiency for DNA recovery than DNase I hypersensitive site sequencing(DNase-seq)and micrococcal nuclease sequencing(MNase-seq).ATAC-seq currently is the most popular technique of genome-wide mapping for chromatin accessibility.It provides information on binding regions of transcription factors and nucleosome localization on the chromatin.Thus,ATAC-seq is of great significance for studying the epigenetics and molecular mechanisms in chromatin structure.In this review,we compare the advantages and disadvantages of multiple techniques for profiling chromatin accessibility,and summarize the principles,main process,development and applications of ATAC-seq.We hope this review will provide a reference for study of genome-wide mapping for chromatin accessibility,identification of cis-regulatory elements,and dissection of the epigenetic and genetic regulatory networks using the ATAC-seq technology in eukaryotes.

关 键 词：染色质转座酶可及性测序 染色质开放区域 Tn5转座酶 表观遗传修饰 转录因子 

分 类 号：Q343.2[生物学—遗传学]