广西辣椒病毒病调查及病原种类初步鉴定  

Investigation and Preliminary Identi fication of Pathogeny Species of Pepper VirusDisease in Guangxi

作　　者：龚明霞[1,2] 赵虎 王萌[1] 吴星[1] 赵曾菁[1] 黄金梅 何志 王日升[1] GONG Ming-xia;ZHAO Hu;WANG Meng;WU Xing;ZHAO Zeng-jing;HUANG Jin-mei;HE Zhi;WANG Ri-sheng(Vegetable Research Institute,Guangxi Academy of Agricultural Sciences,Nanning530007,Guangxi,China;Guangxi Key Laboratory of Vegetable Breeding and New Technology Development,Nanning 53007,Guangxi,China)

机构地区：[1]广西农业科学院蔬菜研究所,广西南宁530007 [2]广西蔬菜育种与新技术研究重点实验室,广西南宁530007

出　　处：《中国蔬菜》2020年第4期74-79,共6页China Vegetables

基　　金：广西科技重大专项(桂科AA17204026);广西农业科学院基本科研业务专项(桂农科2019M30);广西农业科学院科技发展基金项目(桂农科2017JM48);广西自然科学基金项目(2019GXNSFAA185039)。

摘　　要：对广西9个市县辣椒病毒病的发生情况进行初步调查,并采用双抗体夹心酶联免疫吸附法(DAS-ELISA)鉴定75份辣椒疑似病毒病样本的8种常见蔬菜病毒。结果表明,桂南、桂中地区辣椒病毒病发生普遍较桂西、桂北地区的严重。共有71份辣椒样本检测呈阳性,其中辣椒脉斑驳病毒(Chilli veinal mottle virus,Chi VMV)的侵染最普遍,总检出率最高,达63.38%,为广西辣椒的优势病原种类;甜椒叶脉斑驳病毒(Pepper vein mottle virus,PVMV)、黄瓜花叶病毒(Cucumber mosaic virus,CMV)、辣椒轻斑驳病毒(Pepper mild mottle virus,PMMo V)、辣椒环斑病毒(Chilli ring spot virus,Chi RSV)的侵染也很普遍,总检出率分别为56.34%、43.66%、35.21%和33.80%。在71份阳性样本中,有87.32%的样本受2种及2种以上病毒的复合侵染,受2种或3种病毒复合侵染的分别占32.39%和29.58%;2种病毒复合侵染类型中,以ChiVMV+CMV、PVMV+PMMo V复合侵染最普遍,均占21.74%;3种病毒复合侵染类型中,以Chi VMV+CMV+PVMV复合侵染最普遍,占23.81%。This paper conducted initial investigation on occurrence situation of pepper virus disease in 9 cities and counties of Guangxi,and identi fied 8 kinds of common vegetable viruses from 75 suspected infected pepper samples by double antibody sandwich enzyme-linked immunosorbent assay( DAS-ELISA).The results showed that the virus diseases occurred in southern and centre Guangxi were generally more severe than in western and north Guangxi.There were 75 pepper samples reacting positive,among them the infection of Chilli veinal mottle virus( ChiVMV) was very common.The total detection rate was the highest,reaching 63.38%.It was the prevalent pathogenic virus in Guangxi.The detection rates of Pepper vein mottle virus (PVMV),Cucumber mosaic virus( CMV),Pepper mild mottle virus( PMMoV) and Chilli ring spot virus (ChiRSV) were 56.34%,43.66%,35.21% and 33.80%,respectively.Among the 75 positive samples, 87.32% samples were mix-infected by over 2 viruses.Among them,the mixed infections by 2 or 3 viruses were 32.39% and 29.58%,respectively.Among the mixed infections by 2 kinds of viruses,ChiVMV+CMV and PVMV+PMMoV were the most common,all holding 21.74%.Among the mixed infections by 3 kinds of viruses,the mixed infection of ChiVMV+CMV+PVMV was the most common,accounting for 23.81%.

关 键 词：辣椒 病毒病 DAS-ELISA 病原鉴定 广西 

分 类 号：S436.418.12[农业科学—农业昆虫与害虫防治]