海雀稗种质资源的耐盐性评价  

Evaluation of Salt Tolerance of Paspalum vaginatum Germplasm Resources

作　　者：申晴[1,2] 韦海燕 卞华 王志勇 丁西朋[2] SHEN Qing;WEI Haiyan;BIAN Hua;WANG Zhiyong;DING Xipeng(Institute of Tropical Crops,Hainan University/Hainan Key Laboratory for Biotechnology of Salt Tolerant Crops,Hainan University,Haikou,Hainan 570228,China;Tropical Crops Genetic Resources Institute,Chinese Academy of Tropical Agricultural Sciences,Haikou,Hainan 571101,China;Key Laboratory of Ministry of Education for Genetics and Germplasm Innovation of Tropical Special Trees and Ornamental Plants,Hainan University,Haikou,Hainan 570228,China)

机构地区：[1]海南大学热带作物学院/海南省耐盐作物生物技术重点实验室,海口570228 [2]中国热带农业科学院热带作物品种资源研究所,海口571101 [3]海南大学热带特色林木花卉遗传与种质创新教育部重点实验室,海口570228

出　　处：《热带生物学报》2020年第1期11-19,共9页Journal of Tropical Biology

基　　金：2017年海南省研究生创新科研课题(Hys2017-81);2018年海南大学作物学学科研究生科研创新课题(ZWCX2018032);滇桂黔石漠化地区特色作物产业发展关键技术集成示范项目;海南省教育厅重点项目(Hnky2019ZD-3);科技基础资源调查专项(2017FY100600)。

摘　　要：对海雀稗耐盐体系进行优化,并通过测定坪用质量和枯黄率对27份海雀稗种质资源进行了耐盐性评价。结果表明,7个不同NaCl浓度(0,5,10,15,20,25,30 g·L^-1)处理下,坪用质量、枯黄率和叶色各指标之间存在显著差异(P<0.05)。随着NaCl处理浓度的增加,坪用质量显著下降(P<0.05),枯黄率显著增加(P<0.05),叶色显著变浅(P<0.05)。建立回归方程,以枯黄率50%为标准,确定海雀稗最适盐处理浓度为25 g·L^-1。利用25 g·L^-1 NaCl对27份海雀稗种质资源进行耐盐性评价,筛选出2种极端耐盐种质:USA17-18(耐盐)和USA17-26(敏盐)。对海雀稗耐盐极端材料的钠钾离子含量进行测定,发现盐处理后,地上和地下Na+含量均显著增加,但K+含量和K/Na值都显著下降。减少Na+的摄入,维持较高的K+含量,可能是海雀稗耐盐的机制。In order to obtain salt-tolerant Paspalum vaginatum Germplasm resources,the Salt tolerance system of Paspalum vaginatum was optimized and used to evaluate the salt tolerance of 27 accessions of P.vaginatum.The accessions were treated with NaCl at 7 levels of concentration(0,5,10,15,20,25 and 30 g·L^-1)and their turf quality,leaf firing and leaf color were measured.The results showed that there were significant differences in the indicators of turf quality,leaf firing and leaf colour(P<0.05)between the P.vaginatum accessions under the NaCl treatments.With the increase of NaCl concentration,the P.vaginatum accessions were significantly higher in turf quality(P<0.05)and leaf firing(P<0.05),and significantly paler in leaf colour(P<0.05).A regression equation was established to determine the optimum salt treatment concentration for P.vaginatum at25 g·L^-1 with the leaf firing of 50%as a criterion.The NaCl concentration of 25 g·L^-1 was used to evaluate the salt tolerance of 27 accessions of P.vaginatum,based on which two accessions,USA17-18 with very high salt tolerance and USA17-26 with very low salt tolerance or high salt sensitivity,were selected and their contents of Na+and K+were determined.It was found that the two accessions increased significantly in Na+content above ground and underground after salt treatment,but decreased significantly in K+content and K/Na value,and that the very highly salt tolerant accession was lower in Na+content and higher in K+content as against the very low salt tolerant accession.Reducing Na+intake and maintaining high K+content may be the mechanism of salt tolerance of P.vaginatum.

关 键 词：海雀稗 耐盐性 坪用质量 枯黄率 

分 类 号：S543.9[农业科学—作物学]