ATAF2正调控拟南芥对橡胶树白粉菌的抗病性  

ATAF2 positively contributes to the disease resistance against Oidium heveae in Arabidopsis

作　　者：叶鸿鹰 梅双双[2] 戎伟[2] YE Hongying;MEI Shuangshuang;RONG Wei(College of Life Sciences and Pharmacy,Hainan University,Haikou Hainan,570228,China;College of Plant protection,Hainan University,Haikou Hainan,570228,China)

机构地区：[1]海南大学生命科学与药学院,海口570228 [2]海南大学植物保护学院,海口570228

出　　处：《热带生物学报》2020年第1期58-62,共5页Journal of Tropical Biology

基　　金：海南省自然科学基金(317026);国家自然科学基金(31560296)。

摘　　要：橡胶树白粉菌(Oidium heveae)侵染拟南芥野生型Col-0,激发拟南芥的抗病反应。该抗病反应依赖于EDS1(enhanced disease susceptibility 1),EDS1在TIR-NB-LRR(Toll-Interleukin1 Receptor-nucleotide binding-leucine-rich repeat)类抗性基因介导的抗病信号通路中发挥非常重要的作用,但目前还不清楚具体的EDS1上、下游作用元件是什么。为了进一步研究橡胶树白粉菌在拟南芥上激发的抗疾信号通路,本实验室对接种橡胶白粉菌oidium heveae HN1106的拟南芥野生型Col-0进行了RNA-Seq数据分析,结果表明,拟南芥Col-0在接种橡胶树白粉菌4 d后,NAC家族(ATAF1,2 and CUC2,NAM)转录因子ATAF2基因上调表达20倍,并且正调控拟南芥对橡胶树白粉菌的抗病性。另外,笔者通过体外蛋白质pull-down试验和体内原生质体免疫共沉淀试验,发现ATAF2可以直接与EDS1发生相互作用,这为将来进一步阐明EDS1抗病信号通路奠定了很好的基础。It has been reported that Oidium heveae triggers the disease resistance in model plant Arabidopsis thaliana in an Enhanced Disease Susceptibility 1(EDS1)dependent manner,and EDS1 plays an important role in the signal pathway mediated by Toll-Interliukin 1 Receptor-nucleaotide binding-leucine-rich repeat(TIRNB-LRR)type resistance genes.However,the upstream and downstream signaling of EDS1 is still unknown.In this study,A.thaliana Col-0 was inoculated with O.heveae HN1106,and we found the expression of ATAF2 gene,a member of plant NAC transcription factor family,was up-regulated 20 times after 4 days of inoculation as against 0 day of inoculation.Moreover,ATAF2 positively contributed to the disease resistance to O.heveae in A.thaliana.Furthermore,we found ATAF2 directly interacted with EDS1 in vitro and in vivo by GST pulldown and Co-immunoprecipitation assays,which lays a basis for the future study of EDS1 mediated resistance pathway.

关 键 词：ATAF2 EDS1 拟南芥 橡胶树白粉菌 抗病性 

分 类 号：S763.7[农业科学—森林保护学]