Transient expression of SbDhr2 and MeHNL in Gossypium hirsutum for herbivore deterrence assay with Spodoptera litura  

作　　者：MAHAJAN Chavi NASER Rafiuddin GUPTA Shantikumar 

机构地区：[1]Department of Botany,Maulana Azad College of Arts Scienee and Commerce,Rauza Bagh,Aurangabad,Maharashtra 431001,India [2]Government Institute of Scienee,Aurangabad,Maharashtra 431001,India

出　　处：《Journal of Cotton Research》2020年第1期1-12,共12页棉花研究（英文）

基　　金：funded by the Dept.of Botany,Maulana Azad College of Arts,Science and Commerce,Aurangabad,Maharashtra,India and Government Institute of Science,Aurangabad,Maharashtra,India。

摘　　要：Background:Spodoptera litura(Lepidoptera:Noctuidae),commonly known as tobacco cutworm or cotton leafworm,is a polyphagous pest which causes considerable damage to cotton(Gossypium hirsutum)and other crops.Herbivore-induced defence response is activated in plants against chewing pests,in which plant secondary metabolites play an important role.Dhurrinase2(SbDhr2),a cyanogenicβ-glucosidase from Sorghum bicolor,is the key enzyme responsible for the hydrolysis of dhurrin(cyanogenicβ-glucosidic substrate)to phydroxymandelonitrile.Hydroxynitrile lyase(Me HNL)from Mannihot esculanta catalyses the dissociation of cyanohydrins to hydrogen cyanide and corresponding carbonyl compound,both enzymes play a pivotal role in plant defence mechanism.Results:SbDhr2 and Me HNL genes were expressed individually and co-expressed transiently in cotton leaves.We examined the feeding response of S.litura to leaves in the choice assay.The S.litura population used in this study showed better feeding deterrence to leaves co-expressing both genes compared with the expression of an individual gene.Conclusion:Our results suggest that co-expression of SbDhr2 and Me HNL genes in cotton leaves demonstrate feeding deterrence to S.litura.Engineering cyanogenic pathway in aerial parts of cotton would be an additional defence strategy against generalist pests and can be enhanced against specialist pests.

关 键 词：SbDhr2 MeHNL HCNc(hydrogen CYANIDE concentration) HCNp(hydrogen CYANIDE potential) TRV(tobacco rattle virus) SPODOPTERA litura Plant defence 

分 类 号：S435.622[农业科学—农业昆虫与害虫防治]