神经梅毒合并特殊梅毒疹1例及其梅毒螺旋体基因分型  

One case of neurosyphilis combined with special syphilis rash and its genotype of Treponema pallidum

作　　者：旷旭 周静 邱娇娇 蒋燕萍 陆洪光 申小平 KUANG Xu;ZHOU Jing;QIU Jiaojiao;JIANG Yanping;LU Hongguang;SHEN Xiaoping(Department of Dermatology,the Affiliated Hosptial of Guizhou Medical University,Guiyang,Guizhou 550004,China)

机构地区：[1]贵州医科大学附属医院皮肤科,贵州贵阳550004

出　　处：《徐州医科大学学报》2020年第2期130-132,共3页Journal of Xuzhou Medical University

基　　金：贵州省医学科学重点项目(黔科合LH【2017】7189号)。

摘　　要：目的利用双基因分型法确定1例神经梅毒合并特殊梅毒疹患者所感染的梅毒螺旋体基因型别。方法提取患者外周血中的梅毒螺旋体基因组DNA,利用PCR扩增特异性梅毒螺旋体基因tp47基因,利用PCR扩增arp、tpr两个基因,以arp基因所含重复序列数、tpr基因RFLP基因型别联合确定该患者所感染的梅毒螺旋体基因型别。结果PCR扩增出tp47基因片段。双基因分型法鉴定该梅毒螺旋体分型为14d。结论该患者感染的梅毒螺旋体基因型为14d,该型别与神经梅毒的易感性具有明显的相关性,该患者特殊的临床表现可能与该基因型有关。Objective To determine the genotype of Treponema pallidum infected in a neurosyphilis patient with special syphilis rash.Methods The peripheral blood samples were collected from the patient to obtain the genomic DNA of Treponema pallidum.PCR was used to amplify the specific gene tp47 of Treponema pallidum.The genes arp and tpr were amplified by PCR.The number of repeated fragments within gene arp and the RFLP genotype of gene tpr were adopted to determine the genotype of Treponema pallidum.Results The fragments of gene tp47 gene were amplified by PCR.Based on the double genotyping method,the genotype of Treponema pallidum was identified as 14d.Conclusions The genotype of Treponema pallidum infected in the patient is 14d,which is associated with the susceptibility of neurosyphilis.This type has a significant correlation with the susceptibility of neurosyphilis.The patient's special clinical manifestations may be related to the genotype.

关 键 词：梅毒 梅毒螺旋体 CDC基因分型 

分 类 号：R759.1[医药卫生—皮肤病学与性病学]