SSR、SFP、InDel和ILP标记在不同抗瘟性水稻品种中的应用比较  

Comparision of SSR, SFP, InDel and ILP Markers Apply to Different Blast Resistant Rice Varieties

作　　者：杨漫[1,2] 关列梅 曾红丽 张露倩 于玉凤 王悦[1,2] Yang Man;Guan Liemei;Zeng Hongli;Zhang Luqian;Yu Yufeng;Wang Yue(College of Agronomy,Hunan Agricultural University,Changsha,410128;Rice and Rapeseed Disease Resistance Breeding Key Laboratory of Hunan Province,Hunan Agricultural University,Changsha,410128)

机构地区：[1]湖南农业大学农学院,长沙410128 [2]湖南农业大学,水稻油菜抗病育种湖南省重点实验室,长沙410128

出　　处：《分子植物育种》2020年第7期2244-2249,共6页Molecular Plant Breeding

基　　金：国家自然科学基金(31301650);国家重点研发计划(2017YFD0301501);湖南省大学生创新性实验计划项目(XCX17083);湖南农业大学作物种质创新与资源利用国家重点实验室培育基地开放课题(16KFXM12);湖南省自然科学基金(2017JJ3113);湖南省教育厅优秀青年项目(16B127)共同资助。

摘　　要：利用SSR、SFP、InDel和ILP标记对水稻品种进行通用性分析,明确其PCR扩增的有效性和多态性,以期为分子标记辅助选择的应用效果评价奠定基础。结果表明:选用1678对标记对TY和CO39进行PCR扩增,有扩增产物的标记为1578对,扩增产物率为94.04%,其中,两亲本间共检测到133对多态标记,多态检出率为10.34%。4种分子标记间比较发现,SSR标记具有数量多、染色体覆盖度广等特点,而ILP标记的多态检出率最高,达28.90%,分别是SSR、SFP和In Del标记的2.79、4.77、1.47倍。因此,在水稻育种实践中,将SSR和ILP标记结合用于基因型筛选,可以提高选择效率,加快育种进程。To determine the effectiveness and polymorphism of the PCR amplification,SSR,SFP,InDel and ILP markers were used to analyze the universality of rice varieties to lay a foundation for the evaluation of the application effect of molecular marker-assisted selection in the study.The results showed that 1678 pairs of primers were used for PCR amplification of TY and CO39.The amplified product was labeled as 1578 pairs,and the amplified product rate was 94.04%.Among them,133 pairs of polymorphic markers were detected between the two parents and the polymorphic detection rate was 10.34%.Comparison of the four molecular markers found that SSR markers had a large number and wide chromosome coverage.The polymorphism detection rate of ILP markers was the highest,which was 28.90%that were 2.79,4.77 and 1.47 times of SSR,SFP and InDel markers,respectively.Therefore,the combination of SSR and ILP markers for genotype screening could improve the selection efficiency and accelerate the breeding process in rice breeding practice.

关 键 词：水稻 分子标记 通用性 多态性 

分 类 号：S511[农业科学—作物学]