褐藻寡糖用于海水小球藻和盐生杜氏藻异养培养及其促生长作用机制  

Heterotrophic Culture of Chlorella sp.and Dunaliella salina Using Alginate Oligosaccharide and Its Growth Promoting Mechanism

作　　者：马冬冬[1] 李永富[2] MA Dong-Dong;LI Yong-Fu(The Key Laboratory of Marine Environment and Ecology,Ministry of Education,Ocean University of China,Qingdao 266100,China;CAS Key Laboratory of Experimental Marine Biology,Center for Ocean Mega-Science,Institute of Oceanology,Chinese Academy of Sciences,Qingdao 266071,China)

机构地区：[1]中国海洋大学海洋环境与生态教育部重点实验室,山东青岛261000 [2]中国科学院海洋研究所实验海洋生物学重点实验室,山东青岛266071

出　　处：《中国海洋大学学报（自然科学版）》2020年第5期40-45,共6页Periodical of Ocean University of China

基　　金：山东省自然科学基金项目(ZR2017QC008)资助。

摘　　要：为评价酶解褐藻寡糖对海水小球藻(Chlorella sp.)和盐生杜氏藻(Dunaliella salina)的促生长作用。本文测定了50 mg·L^-1质量浓度褐藻寡糖用量对2种微藻的比生长速率、色素含量、光合放氧、呼吸耗氧速率和叶绿素荧光参数的影响。研究表明:光照下添加褐藻寡糖,2种微藻的细胞生长速率均明显提升,海水小球藻在兼养下的比生长速率分别是自养和异养下的2.4和2.6倍;而盐生杜氏藻在兼养下的比生长速率达0.259 d^-1,是自养的1.9倍,异养的1.3倍。2种微藻均可利用寡糖进行异养生长,且在暗处利用褐藻寡糖作为有机碳源获得的生长速率与自养接近。异养下海水小球藻的呼吸耗氧速率显著高于自养和兼养,而盐生杜氏藻的呼吸耗氧速率在兼养下最高,自养下最低。兼养条件下,盐生杜氏藻的叶绿素含量、光合放氧速率、最大光化学效率F v/F m和光化学淬灭qP均显著高于自养条件,但海水小球藻未见显著变化。研究结果表明,褐藻寡糖促进海水小球藻生长主要通过提高其异养同化作用实现;促进盐生杜氏藻生长主要通过提高其异养同化和光合作用实现。The growth-promoting effect of alginate oligosaccharide(ADO)on Chlorella sp.and Dunaliella salina was evaluated in this study.The specific growth rate,pigment content,photosynthetic oxygen evolution,respiratory oxygen consumption rate and chlorophyll fluorescence parameters of two microalgae under 50 mg·L^-1 ADO added were determined.The results showed that the ADO significantly promoted the growth of two microalgae under light conditions.The specific growth rate of Chlorella sp.under mixtrophic condition was 2.4 times and 2.6 times higher than that under autotrophic and heterotrophic conditions,respectively.While the specific growth rate of D.salina under mixtrophic condition was 0.259 d^-1,which was 1.9 times of that under autotrophic condition and 1.3 times of that under heterotrophic condition.Both microalgae can use ADO for heterotrophic growth,and the growth rate obtained by using ADO as organic carbon source in the dark was close to that of autotrophic condition.The respiratory oxygen consumption rate of Chlorella sp.in heterotrophic culture was significantly higher than that in autotrophic and mixtrophic cultivations while that of D.salina was the highest in mixtrophic cultivation and the lowest in autotrophic cultivation.The chlorophyll content,photosynthetic oxygen evolution rate and chlorophyll fluorescence parameters,i.e.the maximal photochemical efficiency of PSII(F v/F m)and photochemical quenching(qP),of D.salina in mixtrophic cultivation were all significantly higher than those in autotrophic cultivation;however,those of Chlorella sp.slightly changed in comparison with control.ADO promoted the growth of Chlorella sp.by enhancing the heterotrophic assimilation,and increased the growth of D.salina by improving both the heterotrophic assimilation and photosynthesis process.

关 键 词：褐藻寡糖 海水小球藻 盐生杜氏藻 促进生长 异养培养 

分 类 号：Q591[生物学—生物化学]