NH4-N氮源下海洋酸化对东海原甲藻和米氏凯伦藻生长的影响  

Effects of Ocean Acidification on the Growth of Prorocentrum donghaiense and Karenia mikimotoi under NH4-N Source

作　　者：贺云凤 逄凯 李克强[1] 梁生康[1] 王修林[1] HE Yun-Feng;PANG Kai;LI Ke-Qiang;LIANG Sheng-Kang;WANG Xiu-Lin(The Key Laboratory of Marine Chemistry Theory and Technology,Ministry of Education,College of Chemistry and Chemical Engineering,Ocean University of China,Qingda O266100,China)

机构地区：[1]中国海洋大学海洋化学理论与工程技术教育部重点实验室,山东青岛266100

出　　处：《中国海洋大学学报（自然科学版）》2020年第5期94-103,共10页Periodical of Ocean University of China

基　　金：国家重点基础研究发展计划项目(2017YFC1404300);国家自然科学基金项目(41676062,U1606404)资助。

摘　　要：本文选取东海原甲藻和米氏凯伦藻,以氨氮(NH4-N)为氮源,通过增加p CO2(800和1500μatm)改变海水的酸化程度,研究海洋酸化对东海原甲藻和米氏凯伦藻生长以及抗氧化生物酶活性的影响。研究结果表明,海洋酸化对东海原甲藻和米氏凯伦藻的生长均有抑制作用,两种藻的生长速率在酸化条件下均低于正常海水条件。东海原甲藻和米氏凯伦藻的最大氮吸收速率及其与半饱和常数的比值(Vmax/Ks比率)均随着酸化程度的加深而降低,表明酸化抑制了浮游植物对NH4-N的吸收。东海原甲藻在三个实验条件下的Vmax/Ks比率均高于米氏凯伦藻,表明东海原甲藻比米氏凯伦藻有更强的环境适应性。海洋酸化诱导了东海原甲藻和米氏凯伦藻的活性氧(ROS)升高,随着酸化程度加深,抗氧化生物酶—超氧化歧化酶(SOD)和过氧化氢酶(CAT)活性升高,这说明,海洋酸化对两种甲藻生长的抑制,通过抗氧化酶的活性变化在生理层面对外界环境作出响应,与生长和营养盐吸收过程相对应。We studied the effects of ocean acidification elevated p CO2(800 and 1500μatm)on the growth and antioxidant enzyme activities of two dinoflagellates Prorocentrum donghaiense and Karenia mikimotoi under NH4-N source.Ocean acidification inhibited the growth of P.donghaiense and K.mikimotoi.The growth rates of two dinoflagellates were lower under lower pH levels.Ocean acidification reduced the nitrogen uptake rates and ratios of nitrogen uptake rates to half-saturation coefficient(V max/K s)of P.donghaiense and K.mikimotoi.The V max/K s ratios of P.donghaiense were higher than those of K.mikimotoi,which implied that the highly competitive position of P.donghaiense under NH4-N source.Ocean acidification induced reactive oxygen species(ROS)based on the results that the maximum enzyme activities of superoxide dismutase(SOD)and catalase(CAT)increased under lower pH levels.The effects of ocean acidification on the growth of P.donghaiense and K.mikimotoi were reflected physiologically by the activities of SOD and CAT.

关 键 词：海洋酸化 NH4-N 东海原甲藻 米氏凯伦藻 生长吸收动力学 生物酶活性 

分 类 号：P76[天文地球—海洋科学]