投喂频率对池塘工程化循环水养殖大口黑鲈生长、生理及肝脏GH、IGF-I基因表达丰度的影响  

Effects of feeding frequency on the growth,physiology and expression abundance of IGF-I and GH mRNA in largemouth bass(Micropterus salmoides)reared in in-pond raceway culture systems

作　　者：谢苏明 王裕玉 聂志娟[2] 孙毅 徐钢春 XIE Suming;WANG Yuyu;NIE Zhijuan;SUN Yi;XU Gangchun(College of Fisheries and Life Science,Shanghai Ocean University,Shanghai 201306,China;Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization,Ministry of Agriculture and Rural Affairs,Freshwater Fisheries Research Center,Chinese Academy of Fishery Sciences,Wuxi 214081,China)

机构地区：[1]上海海洋大学水产与生命学院,上海201306 [2]中国水产科学研究院淡水渔业研究中心,农业农村部淡水渔业和种质资源利用重点实验室,江苏无锡214081

出　　处：《中国水产科学》2020年第4期363-374,共12页Journal of Fishery Sciences of China

基　　金：国家现代化农业特色淡水鱼产业技术体系(CARS-46);江苏省农业科技自主创新基金(CX(16)1004);中央级公益性科研院所基本科研业务费项目(2018JBFR01).

摘　　要：为研究池塘工程化循环水养殖模式下投喂频率对大口黑鲈(Micropterus salmoides)生长、生理及肝脏生长激素基因(GH)、类胰岛素生长因子-I基因(IGF-I)相对表达丰度的影响,选用初始体重为(5.0±0.4)g的大口黑鲈为研究对象,设置3个投喂频率组(2次/d、3次/d、4次/d),每组3个重复,开展为期120 d的养殖试验。结果表明,第30天和第60天时,大口黑鲈末体重、增重率和特定生长率均未受到投喂频率的显著影响(P>0.05),而在第90天时,2次/d组试验鱼的生长显著高于4次/d组(P<0.05),与3次/d组相比无显著性差异(P>0.05);第120天时,2次/d组试验鱼的生长显著高于3次/d组和4次/d组(P<0.05);投喂频率对血清谷氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)、碱性磷酸酶(ALP)活性、溶菌酶、皮质醇和甘油三酯(TG)含量、肝脏总抗氧化能力(T-AOC)、谷胱甘肽过氧化物酶(GSH-Px)活性及丙二醛(MDA)含量均无显著性影响(P>0.05)。随着投喂频率的增加,肝脏过氧化氢酶(CAT)活性及血清总蛋白(TP)、血糖(Glu)含量呈降低的趋势且血清总胆固醇(TC)呈上升的趋势;第30天时投喂频率对肝脏总超氧化物歧化酶(T-SOD)活性无显著性影响,而第60天、第90天和第120天时,4次/d组T-SOD活性显著低于2次/d组(P<0.05)。第30天、第60天和第120天时,2次/d组肝脏IGF-I基因的相对表达量最高(P<0.05),第90天时各组间肝脏IGF-I相对表达量无明显差异(P>0.05);第30天和第60天时,肝脏GH基因相对表达量未受到投喂频率的影响(P>0.05),而第90天和第120天时4次/d组肝脏GH基因相对表达量显著低于2次/d组(P<0.05)。因此,基于大口黑鲈的生长性能、生理效应及肝脏GH、IGF-I基因表达丰度的综合考虑,池塘工程化循环水养殖模式下初始体重为(5.0±0.4)g的大口黑鲈适宜投喂频率为2次/d。A 120-day experiment was conducted to investigate the effects of different feeding frequencies on the growth,physiology,and hepatic growth hormone(GH)and insulin-like growth factor-I(IGF-I)gene expression levels of Micropterus salmoides(body weight:5.0 g±0.4 g)reared in in-pond raceway culture systems(IPRS).Fish were ran-domly assigned to one of three feeding frequencies(2,3 and 4 times/d)following the same ration size.No significant differences were observed in final body weight,weight gain or specific growth rate of fish fed at different feeding fre-quencies on days 30 and 60(P>0.05).Fish fed 2 times/d showed higher growth than those fed 4 times/d on day 90,and had higher growth than those fed 3 times/d and 4 times/d on day 120.No significant differences were found in serum alanine aminotransferase(ALT),aspartate transaminase(AST),or alkaline phosphatase(ALP)activities,lysozyme,cortisol and triglyceride(TG)content,hepatic total antioxidant capacity(T-AOC),glutathione peroxidase(GSH-Px)activities,nor in malondialdehyde(MDA)content among fish fed at different feeding frequencies(P>0.05).Serum to-tal protein(TP)and glucose(Glu)content,and catalase(CAT)activity,decreased,while total cholesterol(TC)content increased with increasing feeding frequency.Fish fed 2 times/d had higher hepatic total superoxide dismutase(T-SOD)than those fed 4 times/d on days 60,90 and 120(P<0.05).Fish fed 2 times/d exhibited higher IGF-I gene mRNA ex-pression on days 30,60 and 120(P<0.05),while no significant difference was found on day 90.No significant differ-ence was found in GH gene mRNA expression on days 30 and 60,while fish fed 4 times/d exhibited lower GH gene mRNA expression than those fed 2 times/d on days 90 and 120(P<0.05).Based on the growth,physiology,and hepatic GH and IGF-I gene expression levels,the optimal feeding frequency for Micropterus salmoides(average body weight:5.0 g±0.4 g)reared in IPRS is 2 times/d.These data are crucial for the optimization of culture conditions and feeding management strategies in IPRS cultu

关 键 词：大口黑鲈 池塘工程化循环水养殖模式 投喂频率 生长 生理 基因表达丰度 

分 类 号：S96[农业科学—水产养殖]