化学计量学结合信息熵赋权优选半夏白术天麻汤提取工艺  

Optimization of extraction technology of Banxia Baizhu Tianma Decoction by chemometrics combined with information entropy weighting

作　　者：徐男[1] 孙蓉[2] 崔焕月 黄欣 李晓宇[1] 王亮[1] 时海燕 XU Nan;SUN Rong;CUI Huan-yue;HUANG Xin;LI Xiao-yu;WANG Liang;SHI Hai-yan(Shandong Research Academy of Traditional Chinese Medicine,Jinan 250014,China;Institute of Advanced Medical Sciences,Shandong University,Jinan 250012,China;The First Hospital Affiliated to Shandong First Medical University,Jinan 250014,China)

机构地区：[1]山东省中医药研究院,山东济南250014 [2]山东大学高等医学研究院,山东济南250012 [3]山东第一医科大学第一附属医院,山东济南250014

出　　处：《中草药》2020年第4期995-1002,共8页Chinese Traditional and Herbal Drugs

基　　金：山东省中医药科技发展计划项目(2019-0368);山东省中医药科技发展计划项目(2017-136);山东省重点研发计划项目(2017CXGC1308);山东省泰山学者工程专项(ts201511107)。

摘　　要：目的建立化学计量学与信息熵赋权相结合的提取工艺优化方法,并将其应用于半夏白术天麻汤水提工艺优化,以充分保证经典名方的有效性和质量一致性。方法以半夏白术天麻汤为模型药物,采用HPLC法建立指纹图谱,对共有峰峰面积进行主成分分析(PCA),以PCA总因子得分、指纹图谱相似度及干膏收率为评价指标,采用L9(34)正交设计法考察加水量、浸泡时间、煎煮时间、煎煮次数对提取效果的影响,信息熵赋权法确定各指标的客观权重,优化水提工艺参数。结果通过中药色谱指纹图谱相似度评价系统匹配26个共有峰,并确定其中7个化合物。根据综合评分结果,确定最佳提取工艺为12倍加水量,煎煮2次,每次1 h。3批验证综合评分均值为0.4180,RSD为3.32%。结论优选后的工艺提取率高、稳定性和重复性好,适用于半夏白术天麻汤经典名方制剂的工业化生产。Objective The extraction process optimization method of chemometrics combined with information entropy weight was established and applied to the water extraction process optimization of Banxia Baizhu Tianma Decoction(BBTD), in order to fully ensure the effectiveness and quality consistency of classical prescription. Methods Taking BBTD as the model drug, the fingerprint was established by HPLC method. The common peak area was analyzed by principal component analysis(PCA), the total factor score of PCA, the similarity of fingerprint and the yield of dry paste were used as evaluation indexes. L9(34) orthogonal design was used to investigate the effects of adding water, soaking time and boiling time on the extraction effect, and the objective weight of each index was determined by information entropy weighting method. The technological parameters of water extraction were optimized. Results Twenty-six common peaks and seven compounds were identified by the similarity evaluation system of chromatographic fingerprints of traditional Chinese medicine. According to the results of comprehensive score, the optimum extraction process of the preparation was determined to be 12 times the amount of water, decoction twice, 1 hour each time. The average comprehensive score of the three batches of verification was 0.418 0 with RSD of 3.32%. Conclusion The optimized process has high extraction rate, good stability and repeatability, and is suitable for the industrial production of BBTD.

关 键 词：半夏白术天麻汤 信息熵 正交设计 指纹图谱 化学计量学 提取工艺 经典名方 有效性 质量一致性 HPLC 主成分分析 相似度 

分 类 号：R284.2[医药卫生—中药学]