头痛宁滴丸治疗偏头痛的实验研究  

Therapeutic effect of Toutongning Dropping Pills on migraine

作　　者：沈廷明 姜溪[2] 陈芙蓉[2] 只德广[2] 岳南[2] 孙双勇[2] SHEN Ting-ming;JIANG Xi;CHEN Fu-rong;ZHI De-guang;YUE Nan;SUN Shuang-yong(Fujian Province Ningde Hospital of Traditional Chinese Medicine,Ningde 352100,China;New Drug Evaluation Co.,Ltd.,Tianjin Institute of Pharmaceutical Research,Tianjin 300301,China)

机构地区：[1]福建省宁德市中医院,福建宁德352100 [2]天津药物研究院新药评价有限公司,天津300301

出　　处：《中草药》2020年第4期1016-1023,共8页Chinese Traditional and Herbal Drugs

摘　　要：目的采用多种实验模型评价头痛宁滴丸治疗偏头痛的有效性。方法采用硝酸甘油致大鼠实验性偏头痛模型,观察头痛宁滴丸对偏头痛的治疗作用;采用小鼠化学性刺激、热刺激及大鼠机械性刺激、福尔马林刺激实验模型,观察头痛宁滴丸的镇痛作用;采用硝酸士的宁致小鼠惊厥实验模型、正常小鼠自主活动实验模型,观察头痛宁滴丸的镇静作用;采用高黏滞血症大鼠模型、微循环障碍小鼠耳廓微循环实验模型,观察头痛宁滴丸的活血作用。结果与模型组比较,头痛宁滴丸给药5d,1、2g/kg剂量能明显减少大鼠挠头次数、爬笼次数,缩短耳红消退时间;头痛宁滴丸1.5、3g/kg剂量明显减少醋酸引起的小鼠扭体次数;对热刺激模型无明显影响;头痛宁滴丸1、2 g/kg剂量能明显提高机械性刺激致痛大鼠的痛阈值,缩短福尔马林致痛大鼠实验II相时间;头痛宁滴丸1.5、3g/kg剂量能明显延长小鼠惊厥潜伏期、减少自主活动次数;头痛宁滴丸0.5、1、2 g/kg剂量明显抑制大鼠全血黏度的升高;1.5、3 g/kg剂量明显改善微循环障碍小鼠耳廓微循环的血液流态、减少红细胞聚集、增加毛细血管交点数。结论头痛宁滴丸有明显的镇痛、镇静、活血作用,对实验性偏头痛有明显的治疗作用。Objective To evaluate the therapeutic effect of Toutongning Dropping Pills in the treatment of migraine in various experimental models. Methods The experimental model of nitroglycerin-induced migraine in rats was used to observe the preventive effect of Toutongning Dropping Pills. The analgesic effect of Toutongning Dropping Pills was observed by chemical stimulation, thermal stimulation in mice, mechanical stimulation and formalin stimulation in rats. The sedative effect of Toutongning Dropping Pills was observed by using the mice convulsion model induced by strychnine nitrate and the model of spontaneous activity in normal mice. The blood-activating effects of Toutongning Dropping Pills were observed in rats with hyperviscosity and mice with microcirculation disorder. Results Five days after Toutongning Dropping Pills were given, compared with the model group, 1 g/kg and 2 g/kg drug dose group significantly reduced the number of climbing cage and scratching head in migraine rats, and shortened the fading time of pink ears. Toutongning Dropping Pills 1.5 g/kg and 3 g/kg dose group significantly reduced the number of writhing induced by acetic acid in mice;While it had no obvious effect on the thermal stimulation model. Toutongning Dropping Pills 1 g/kg and 2 g/kg dose group could significantly improve pain threshold of the mechanical stimulation of rats, and shorten the phase II time of formalin pain in rats;1.5 g/kg and 3 g/kg drug dose group could significantly prolong the incubation period of convulsion and reduce the number of spontaneous activities in mice;0.5 g/kg and 1 g/kg and 2 g/kg drug dose group significantly inhibited the increase of whole blood viscosity in rats;1.5 g/kg and 3 g/kg drug dose group significantly improved the blood flow pattern in mice, reduced erythrocyte aggregation, and increased the number of capillary intersection points. Conclusion Toutongning Dropping Pills have obvious analgesic, sedative, blood-activating functions, and have therapeutic effects on experimental migraine.

关 键 词：头痛宁滴丸 硝酸甘油 偏头痛 镇痛 镇静 血液流变学 活血作用 

分 类 号：R285.5[医药卫生—中药学]