黄根多糖对大鼠矽肺纤维化的作用  

Anti-fibrosis effects of polysaccharide of Prismatomeris tetrandra on silicosis rats

作　　者：陆青兰 黄世稳 韦雪平 周海东 刘桂成 覃慧盈 姚鸿飞 LU Qing-lan;HUANG Shi-wen;WEI Xue-ping;ZHOU Hai-dong;LIU Gui-cheng;QIN Hui-ying;YAO Hong-fei(School of Preclinical Medicine,Youjiang Medical University for Nationalities,Baise 533000,China;The Affiliated Hospital of Youjiang Medical University for Nationalities,Baise 533000,China;School of Clinical Medicine,Youjiang Medical University for Nationalities,Baise 533000,China;School of Public Health and Management,Youjiang Medical University for Nationalities,Baise 533000,China)

机构地区：[1]右江民族医学院基础医学院,广西百色533000 [2]右江民族医学院附属医院,广西百色533000 [3]右江民族医学院临床医学院,广西百色533000 [4]右江民族医学院公共卫生与管理学院,广西百色533000

出　　处：《中草药》2020年第4期1031-1036,共6页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金资助项目(81660694);广西自然科学基金资助项目(2015JJAA40399);广西教育厅重点项目(ZD2014100)。

摘　　要：目的探讨黄根多糖对大鼠矽肺纤维化的作用。方法采用口咽法建立大鼠矽肺模型,将40只雄性成年Wistar大鼠随机分为对照组和造模组,造模组大鼠滴注50.0 mg/mL SiO2水混悬液1.00 mL制备矽肺纤维化模型。造模成功后将造模组随机分为模型组及黄根多糖低、中、高剂量(125、250、500mg/g)组,每组8只。黄根多糖低、中、高剂量组分别ig给予相应药物,对照组和模型组ig给予等量生理盐水,连续给药56d后处死大鼠,采集肺组织。HE、Masson染色检测大鼠肺组织病理学变化;免疫荧光定量PCR(qRT-PCR)检测肺组织中上皮性钙黏附蛋白(E-cadherin)、α平滑肌肌动蛋白(α-SMA)m RNA表达;Western blotting法检测肺组织中E-cadherin、α-SMA、波形纤维蛋白(Vimentin)表达。结果各组大鼠体质量差异不显著;与对照组比较,模型组大鼠肺泡炎症、肺纤维化程度加重,肺脏指数增高,α-SMAm RNA和蛋白表达水平显著升高,E-cadherin mRNA和蛋白表达水平显著降低,Vimentin蛋白表达水平显著升高。与模型组比较,黄根多糖各剂量组大鼠肺泡炎症、肺纤维化程度均下降,肺脏指数均降低,E-cadherinm RNA和蛋白表达水平显著升高,α-SMA m RNA和蛋白表达水平显著降低,Vimentin表达水平显著降低。结论黄根多糖能减轻矽肺大鼠的肺脏器损伤、减缓肺纤维化进程,具有抗矽肺纤维化作用。Objective To investigate the anti-fibrosis effects of polysaccharide of Prismatomeris tetrandra on the silicosis rats and the underlying mechanism. Methods The experimental silicosis rat model was established by oropharyngeal aspiration method. Forty male adult Wistar rats were randomly divided into two groups: saline group and modeling group. The experimental fibrosis of silicosis was induced by dripping 1 mL of 50 mg/mL SiO2 onto the oropharynx of rats of the modeling group. After modeling, the modeling group were randomly divided into one model group with SiO2 and three polysaccharide groups with SiO2 plus low, medium, and high dose of polysaccharide of P. tetrandra, eight rats per group. One day after modeling, rats in three polysaccharide groups were administered with the polysaccharide at a dose of 125, 250, and 500 mg/kg, respectively, daily for 56 d, meanwhile, the saline group and the model group were given the same amount of saline daily for 56 d. The rats were then sacrificed at the 56 th day of the experiment and lung tissues were collected. The pathological changes and fibrosis of the lung tissues were observed by HE staining and Masson staining. Real-time quantitative PCR method was used to detect the mRNA expression of epithelial calcium adhesion protein(E-cadherin) and alpha smooth muscle actin(α-SMA) in lung tissue;Western blotting method was used to measure the levels of E-cadherin, α-SMA, and Vimentin in lung tissue. Results There were no significant differences in body weight between groups. Compared with the saline group, the alveolar inflammation and the pulmonary fibrosis were aggravated, the lung/body coefficient was increased, the levels of α-SMA mRNA and α-SMA protein were significantly increased, the mRNA and protein expression level of E-cadherin was decreased markedly, and the level of Vimentin was increased significantly in the model group. Compared with the model group, the alveolar inflammation and the pulmonary fibrosis were alleviated, the lung/body coefficient was decreased,

关 键 词：黄根多糖 矽肺 纤维化 Α平滑肌肌动蛋白 上皮性钙黏附蛋白 波形纤维蛋白 

分 类 号：R285.5[医药卫生—中药学]