萜烯缀合姜黄素类微量成分与姜黄素类成分在不同姜黄属药材中的定性与定量分析  

Qualitative and quantitative analysis of trace element of terpenoid conjugated curcuminoid and curcuminoids in herbal medicine derived from different Curcuma species

作　　者：廖婉[1] 张倩 孙良堃 董锋 刘芳 何宇新 李玲 侯曙光 李锐[1,4] 肖洪涛 LIAO Wan;ZHANG Qian;SUN Liang-kun;DONG Feng;LIU Fang;HE Yu-xin;LI Ling;HOU Shu-guang;LI Rui;XIAO Hong-tao(State Key Laboratory of Traditional Chinese Medicine Resources in Southwest China,Chengdu University of Traditional Chinese Medicine,Chengdu 611137,China;School of Food and Bioengineering,Xihua University,Chengdu 610039,China;Taiji Group,Chongqing 401147,China;Chengdu Phyto Cosmos Biotechnology Co.,Ltd.,Chengdu 610041,China;Department of Pharmacy,Sichuan Cancer Hospital&Institute,Affiliated Hospital of University of Electronic Science and Technology of China,Chengdu 610072,China)

机构地区：[1]成都中医药大学西南特色中药资源国家重点实验室,四川成都611137 [2]西华大学食品与生物工程学院,四川成都610039 [3]太极集团,重庆401147 [4]成都植宇生物科技有限公司,四川成都610041 [5]电子科技大学附属医院,四川省肿瘤医院药学部,四川成都610072

出　　处：《中草药》2020年第4期1076-1081,共6页Chinese Traditional and Herbal Drugs

基　　金：四川省科技厅科技项目(2016KZ0037,2017NFP0038);西华大学大健康促进中心开放课题(20180420231957);农业部杂粮加工重点实验室开放课题(2019CC02);四川省中医药管理局中医药开发专项(2018KF001);成都中医药大学杏林学者学科人才科研提升计划(XSGG2019005&QNXZ2018026);成都中医药大学科技转化项目(030036005);教育部春晖计划(20191083-127)。

摘　　要：目的对姜黄属药材中萜烯缀合姜黄素类化合物(BCE)进行系统的定性、定量分析。方法利用超高效液相色谱-飞行时间质谱法(UPLC-Q-TOF-MS/MS)建立针对BCE的定性分析,研究其在不同姜黄属药材中的分布情况;再利用UPLC法建立对姜黄、黄丝郁金及蓬莪术中的BCE及3个主要姜黄素(姜黄素、脱甲氧基姜黄素和双脱甲氧基姜黄素)的定量分析,研究BCE在不同姜黄属药材中的含量差异及其与其他姜黄素类成分的含量差异。结果在定性研究中,通过对BCE一级、二级裂解碎片及质谱裂解规律的分析,发现只有姜黄、黄丝郁金中含有该类成分,而蓬莪术中未能检测出BCE;在定量研究中,BCE及3个主要姜黄素在线性范围内均具有良好的线性关系(r≥0.9993);平均回收率在99.407%~113.631%,RSD<4%,测定发现姜黄中的3个姜黄素类成分明显高于另外2种药材,且BCE在3种药材中含量差异较大,姜黄中的BCE含量是黄丝郁金的4倍。结论本研究建立的定性、定量分析方法能够为更多BCE类新颖结构化合物的发现和分析提供借鉴,并为提升姜黄属药材的质量标准提供依据。Objective To qualitatively and quantitatively analyze the terpene-conjugated curcuminoid compoundBisabolocurcumin ether(BCE) in Curcuma species. Methods UPLC-Q-TOF-MS/MS was used to establish a qualitative analysis of BCE, and its distribution in Curcuma species was studied. UPLC was used to establish quantitative analysis of BCE and th ree main curcuminoids in three Curcuma species(Rhizoma Curcumae Longae, HuangsiYujin, Curcuma phaeocaulis), in order to study the difference of BCE content in different Curcuma species and its content difference with other curcuminoids. Results In the qualitative analysis, by analyzing the primary, secondary pyrolysis fragments and the cracking law of BCE, it was found that on ly Jianghuang and HuangsiYujin contained it, but BCE could not be detected in the PengEzhu. In the quantitative analysis, BCE and the three main curcuminoids had good linear relationship in their linear range(r ≥ 0.999 3);The average recovery rates were in range of 99.407%-113.631%, and RSD < 4%. It was found that the content of three curcuminoids in Jianghuang were significantly higher than the other two herbs, and the content of BCE in the three herbs was very different. The BCE content in Jianghuang was four times than that of HuangsiYujin. Conclusion The qualitative and quantitative analysis methods established in this study can provide reference for the discovery and analysis of more novel BCE structure’s compounds, and provide a basis for improving t he quality standards of Curcuma species.

关 键 词：姜黄 黄丝郁金 蓬莪术 UPLC-Q-TOF-MS/MS UPLC 姜黄素 脱甲氧基姜黄素 双脱甲氧基姜黄素 萜烯缀合姜黄素类 

分 类 号：R286.2[医药卫生—中药学]