5株海洋来源真菌的卤盐调节发酵产物分析与代谢产物鉴定  

Analysis of the halogen salts-regulated fermentation products of five marine-derived fungal strains and identification of the metabolite

作　　者：彭雨 朱振虎 周瑞宁 吕学鹏 乔成琳 张慧敏[1] 彭晓娉[1] PENG Yu;ZHU Zhen-hu;ZHOU Rui-ning;LV Xue-peng;QIAO Cheng-lin;ZHANG Hui-min;PENG Xiao-ping(Department of Natural Medicinal Chemistry and Pharmacognosy,School of Pharmacy,Qingdao University,Qingdao 266021,China)

机构地区：[1]青岛大学药学院,天然药物与生药学系,青岛266021

出　　处：《国际药学研究杂志》2019年第12期931-936,945,共7页Journal of International Pharmaceutical Research

基　　金：国家自然科学基金项目(41706077);中国博士后科学基金项目(2019M652309)。

摘　　要：目的对来源于青岛沿海的5株耐盐真菌,分析卤盐胁迫(halogen stress)对次生代谢产物结构多样性和活性的影响,并分离鉴定特征化合物。方法对5株海洋来源真菌进行单菌株多化合物(OSMAC)策略研究,使用高效液相层析(HPLC)法对次生代谢产物化学多样性进行分析,并采用非真菌的紫色色杆菌(Chromobacterium violaceum)进行群体感应(QS)活性测试。用中压柱色谱和制备HPLC进行分离纯化,根据波谱数据鉴定化合物结构。结果发酵培养基中碳氮源或卤盐的变化可改变5株耐盐真菌次生代谢产物的种类,继而带来活性的变化。从菌株L6的大米+NaCl培养基发酵粗提物中分离鉴定其主产物为细交链格孢酮酸。结论卤盐胁迫可丰富耐盐真菌次生代谢产物多样性,海洋来源真菌菌株对药用资源开发具有进一步研究价值。Objective To investigate the effect of halogen stress on the structural diversity and bioactivity of the secondary metabolites,using five marine-derived,halotolerant fungal strains from the coast of Qingdao,and identify major compounds in the metabolites.Methods The one strain-many compounds(OSMAC)strategy was used for investigating the five fungal strains.The chemical diversity of the secondary metabolites was analyzed by high performance liquid chromatography(HPLC),and the quorum sensing(QS)inhibition test was conducted using Chromobacterium violaceum(no fungus).The middle pressure liquid chromatography and preparative HPLC were used for the separation and purification of major metabolite,and the obtained compound was identified according to the spectroscopic data.Results The variation of carbon sources or halogen salts in the fermentation medium could change chemical types of produced secondary metabolites,in the 5 halotolerant fungal strains,which also caused changes in the bioactivity of fermentation extracts.Tenuazonic acid was identified as a major product of the fermentation of L6 strain with the rice+NaCl medium.Conclusion The halogen stress could enrich the diversity of secondary metabolites in halotolerant fungi,and the marine-derived fungal strains are worthy of further investigation to exploit additional medicinal resources.

关 键 词：青岛沿海 耐盐真菌 卤盐胁迫 群体感应抑制活性 单菌株多化合物策略 

分 类 号：R915.1[医药卫生—微生物与生化药学]