曙红Y瑞利散射测定药物中的盐酸美西律  

Determination of mexiletine hydrochloride in drugs by eosin Y Rayleigh light scattering

作　　者：庞向东[1] 向杰 何树华[1] 江虹[1] PANG Xiang-dong;XIANG Jie;HE Shu-hua;JIANG Hong(College of Chemistry and Chemical Engineering,Yangtze Normal University,Chongqing 408100,China)

机构地区：[1]长江师范学院,重庆408100

出　　处：《国际药学研究杂志》2019年第12期950-955,共6页Journal of International Pharmaceutical Research

基　　金：重庆市教委科技基金资助项目(S201910647003);长江师范学院科技基金资助项目(2018CXX111)。

摘　　要：目的建立简便、快速测定药物中盐酸美西律的瑞利散射新方法。方法在酸性Tris-HCl介质及溴代十六烷基吡啶鎓存在下,曙红Y与盐酸美西律以静电引力作用生成具有2个特征散射峰的三元离子缔合物,测定波长为368和586 nm,一定浓度范围的盐酸美西律与缔合物的瑞利散射增强强度(ΔI_(RLS))有线性关系,采用单波长瑞利散射(SWO-RLS)法或双波长瑞利散射(DWO-RLS)法进行测定,依据标准曲线求得盐酸美西律的含量。结果盐酸美西律的线性范围分别为0.005~0.65 mg/L(SWO-RLS法,368 nm)、0.004~0.65 mg/L(SWO-RLS法,586 nm)和0.004~0.65 mg/L(DWO-RLS法,368 nm+586 nm),检出限分别为0.0033 mg/L(SWO-RLS法,368 nm)、0.0040 mg/L(SWO-RLS法,586 nm)和0.0018 mg/L(DWO-RLS法,368 nm+586 nm),样品的加标回收率和相对标准偏差(RSD,n=5)分别为98.6%~103%和1.4%~1.8%(SWO-RLS法,368 nm)。结论本法简便、快速、灵敏、选择性高。Objective To establish a simple,rapid and novel Rayleigh light scattering(RLS)method for rapid determination of mexiletine hydrochloride in drugs.Methods In the presence of acid Tris-HCl medium and cetylpyridinium bromide,eosin Y reacted with mexiletine hydrochloride to form a ternary ion association complex with two characteristic scattering peaks by electrostatic attraction.The detection wavelengths were 368 and 586 nm.There was a linear relationship between the mexiletine hydrochloride concentration in a certain range and the Rayleigh light scattering enhancement intensity(ΔIRLS)of the association complex.Single-wavelength Rayleigh scattering(SWO-RLS)method or dualwavelength Rayleigh light scattering(DWO-RLS)method was used to determine the content of mexiletine hydrochloride,and the mexiletine hydrochloride content was calculated according to the regression equation of standard curve.Results The linear ranges of mexiletine hydrochloride were 0.005-0.65 mg/L(SWO-RLS method,368 nm),0.004-0.65 mg/L(SWO-RLS method,586 nm)and 0.004-0.65 mg/L(DWO-RLS method,368 nm+586 nm),respectisely.Detection limits were 0.0033(SWO-RLS method,368 nm),0.0040(SWO-RLS method,586 nm)and 0.0018 mg/L(DWO-RLS method,368 nm+586 nm),respectisely.The recovery and relative standard deviation(RSD,n=5)for a SWO-RLS method were 98.6-103%and 1.4-1.8%,respectively(SWO-RLS method,368 nm).Conclusion The method is simple,rapid,highly sensitive and high selectie.

关 键 词：曙红Y 溴代十六烷基吡啶鎓 盐酸美西律 单波长 双波长 瑞利光散射 

分 类 号：R917[医药卫生—药物分析学] O657.3[医药卫生—药学]