多杀性巴氏杆菌OmpH对宿主Fn和Plg粘附作用的初步研究  

Preliminrily Charictrization of Host Fibronectin and Plasminogen Binding Activity of Pasteurella Multocida OmpH

作　　者：陈露 朱伟峰[2,3,4] 魏后军 胡波[2,3,4] 仇汝龙 范志宇[2,3,4] 陈萌萌 薛家宾 索朗斯珠[1] 王芳 CHEN Lu;ZHU Wei-feng;WEI Hou-jun;HU Bo;QIU Ru-long;FAN Zhi-yu;CHEN Meng-meng;XUE Jia-bin;Suolang-sizhu;WANG Fang(College of Animal Science,Tibetan Agricultural and Animal Husbandry College,Linzhi 860000,China;Institute of Veterinary Medicine,Jiangsu Academy of Agricultural Sciences,Nanjing 210014,China;Key Laboratory of Veterinary Biological Engineering and Technology,Ministry of Agriculture,Nanjing 210014,China;National Center for Engineering Research of Veterinary Bio-products,Nanjing 210014,China)

机构地区：[1]西藏农牧学院动物科学学院,西藏林芝860000 [2]江苏省农业科学院兽医研究所,江苏南京210014 [3]农业部兽用生物制品工程技术重点实验室,江苏南京210014 [4]国家兽用生物制品工程技术研究中心,江苏南京210014

出　　处：《中国兽医杂志》2019年第10期13-16,19,共5页Chinese Journal of Veterinary Medicine

基　　金：江苏省博士后科研资助计划(2018K035A);中国博士后科学基金面上资助(2018M642196);现代农业产业技术体系建设专项资金(CARS-43-C-1)。

摘　　要：多杀性巴氏杆菌是一种重要的多种动物共患病病原和人兽共患病病原。OmpH是多杀性巴氏杆菌的保护性抗原和宿主细胞粘附因子。很多细菌的粘附因子可以通过粘附Fn等宿主胞外基质分子或血浆纤维蛋白溶解酶原(Plg)从而促进粘附过程,OmpH可能也具有此功能。为了研究OmpH对宿主Fn和Plg的粘附作用,采用原核表达系统表达OmpH,通过Far Western Blot试验和招募抑制试验研究了OmpH对Fn和Plg的结合作用及OmpH多克隆抗体对多杀性巴氏杆菌结合这些分子的抑制作用。结果显示,重组OmpH对Fn和Plg有结合作用,OmpH抗体能够显著抑制多杀性巴氏杆菌对Fn和Plg的粘附。本研究初步证明OmpH能够通过结合宿主Fn和Plg促进多杀性巴氏杆菌的粘附作用,增加了多杀性巴氏杆菌毒力因子和分子致病机制的认识,对巴氏杆菌病防控具有积极意义。Pasteurella multocida is the causative agent of a wide range of disease(pasteurellosis)and a zoonotic pathogen in humans.P.multocida OmpH has been reported to be a protective antigen and an adhesion to host cells.Many bacterial adhesins can adhere to host extracellular matrix(i.e.fibronectin)or plasminogen,which then promote adhesion of the bacteria,and OmpH may play this pathogenic role.The aim of this study was to characterize OmpH as a fibronectin-or plasminogen-binding protein.Here,recombinant OmpH was expressed,and its reaction to recover serum from P.multocida infection was characterized.Then binding activity of OmpH to fibronectin and plasminogen was studied.At last,inhibition activity of OmpH polyclonal antibodies to fibronectin or plasminogen binding activity of P.multocida was characterized.Recombinant P.multocida OmpH was successfully expressed and exhibited apparent reaction to recover serum.Recombinant OmpH exhibited binding activity to fibronectin and plasminogen,and its an-tibodies significantly inhibited the binding activity of P.multocida tobronectin and plasminogen.In conclusion,recombinant OmpH was successfully obtained in this study.It is preliminarily proved that OmpH promotes the adhesion of P.multocida via binding host fibronectin and plasminogen.Our work increases the understanding on virulence factors and molecular pathogenesis of P.multocida,and may have positive effect on the prevention and control of pasteurellosis.

关 键 词：多杀性巴氏杆菌 OmpH 纤维连接蛋白 血浆纤维溶解酶原 

分 类 号：S855.12[农业科学—临床兽医学]