一测多评法同时测定蒙药古日古木-13丸中6种成分  

Simultaneous determination of six components in Mongolia medicine Gurigumu-13 Pills by QAMS

作　　者：邬国栋[1] 郭叶[1] 李刚 张耀升 杨雪苗 马瑞寅 张雨生 张志勇[1] 安明[1] WU Guo-dong;GUO Ye;LI Gang;ZHANG Yao-sheng;YANG Xue-miao;MA Rui-yin;ZHANG Yu-sheng;ZHANG Zhi-yong;AN Ming(Baotou Medical College,Baotou 014060,China;School of Pharmacy,Inner Mongolia Medical University,Hohhot 010100,China)

机构地区：[1]包头医学院,内蒙古包头014060 [2]内蒙古医科大学药学院,内蒙古呼和浩特010100

出　　处：《中草药》2020年第6期1542-1547,共6页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金资助项目(8156068);国家自然科学基金资助项目(21967017);内蒙古自治区科技重大专项:新型治疗高磷酸血症稀土创新药物纳米氢氧化镧临床前药理学研究与开发;内蒙古教育厅大学生创新创业训练计划项目(201910127008);内蒙古教育厅大学生创新创业训练计划项目(201910127011)。

摘　　要：目的建立古日古木-13丸中6种成分没食子酸、羟基红花黄色素A、栀子苷、鞣花酸、木香烃内酯、去氢木香内酯的一测多评方法,验证该方法在古日古木-13丸质量分析中应用的科学性与可行性。方法采用HPLC法,以栀子苷为内参物,建立没食子酸、羟基红花黄色素A、鞣花酸、木香烃内酯、去氢木香内酯的相对校正因子(fs/i),并利用fs/i计算古日古木-13样品中该5种成分的含量,同时采用外标法计算各成分的含量,并比较2种方法差异。结果没食子酸、羟基红花黄色素A、鞣花酸、木香烃内酯、去氢木香内酯的fs/i分别为0.481 0、0.906 4、0.170 9、0.971 2、1.261 5。一测多评法与外标法得到的6批古日古木-13丸样品含量测定结果 RSD<2.0%,没有显著差异。结论以栀子苷为内参物建立fs/i准确,易行,一测多评法可以用于古日古木-13丸中多指标成分的质量评价。Objective To establish a quantitative analysis of multi-components by single marker(QAMS) method for the simultaneous determination of six components of gallic acid, hydroxysafflor yellow A, geniposide, ellagic acid, costunolide and dehydrocostus lactone in Gurigumu-13 Pill, which is proved to be a scientific and feasible method in the quality analysis in Gurigumu-13 Pill. Methods The relative factor(fs/i) of gallic acid, ellagic acid, hydroxysafflor yellow A, costunolide and dehydrocostus lactone were established by HPLC method with geniposide as internal standard, which were used to calculate the content of five constituents in the samples of Gurigumu-13 Pill. Meanwhile, external standard method(ESM) was used to calculate the content of six constituents. The difference between QAMS and ESM was analyzed to evaluate the accuracy of QAMS. Results The fs/i of gallic acid, hydroxysafflor yellow A, ellagic acid, costunolide and dehydrocostus lactone were 0.481 0, 0.906 4, 0.170 9, 0.971 2 and 1.261 5, respectively. The content determination results of six batches of Gurigumu-13 Pill were calculated by the method of QAMS and ESM, with no significant difference in RSD < 2.0%. Conclusion The fs/i established in the QAMS method with geniposide as the internal reference substance is accurate and simple. The QAMS method can be used for the multi-index quality evaluation of Gurigumu-13 Pill.

关 键 词：古日古木-13丸 一测多评法 没食子酸 羟基红花黄色素A 栀子苷 鞣花酸 木香烃内酯 去氢木香内酯 

分 类 号：R286.02[医药卫生—中药学]