基于析因设计的佛手散防治帕金森病作用机制探讨  

Anti-Parkinson mechanism of Foshou Powder based on factorial design

作　　者：曹方引 向菊芳 唐大轩[2] 邓勇[1] 包旭[1] 熊静悦[2] 陈重华[1] 谭正怀[2] CAO Fang-yin;XIANG Ju-fang;TANG Da-xuan;DENG Yong;BAO Xu;XIONG Jing-yue;CHEN Zhong-hua;TAN Zheng-huai(Key Laboratory of Drug-Targeting and Drug Delivery System of Education Ministry,Sichuan Engineering Laboratory for Plant-Sourced Drug and Sichuan Research Center for Drug Precision Industrial Technology,West China School of Pharmacy,Sichuan University,Chendu 610041,China;Sichuan Academy of Chinese Medicine Sciences,Evalution Platform of Pharmacodynamics of TCM for Major Disease Prevention and Treatment,Sichuan Key Laboratory of TCM Quality Evaluation,Chendu 610042,China;School of Pharmacy,Chengdu University of Traditional Chinese Medicine,Chendu 610075,China)

机构地区：[1]四川大学华西药学院,靶向药物与释药系统教育部重点实验室,四川省植物来源工程实验室和四川省小分子药物精准化工程技术研究中心,四川成都610041 [2]四川省中医药科学院,防治重大疾病中药药效学评价平台,四川省中药质量评价重点实验室,四川成都610042 [3]成都中医药大学药学院,四川成都610075

出　　处：《中草药》2020年第6期1559-1566,共8页Chinese Traditional and Herbal Drugs

基　　金：四川省科技厅成果转化项目(2018YSZH0012);四川省科技计划项目(2019JDJQ0040)。

摘　　要：目的探讨佛手散防治帕金森病(PD)的作用及相关机制。方法体内实验采用利血平模型,观察小鼠肛温和运动不能指标,考察当归挥发油和川芎挥发油及其联合应用的影响;体外实验采用荧光分光光度法,以犬尿胺为底物,检测人单胺氧化酶(hMAO)活性和抑制模式,在此基础上,采用2×4析因设计,探讨当归挥发油与川芎挥发油之间的相互作用。结果利血平实验发现联合应用当归挥发油和川芎挥发油可显著改善模型小鼠肛温下降和运动不能;酶活性实验发现当归挥发油和川芎挥发油对h MAO-A和h MAO-B均为竞争性抑制作用,两者配伍使用时对h MAO-A和h MAO-B的活性均有显著的协同增效作用。结论佛手散中的当归、川芎在防治PD中具有显著的协同增效效应,其机制可能与抑制h MAO活性有关。Objective To explore the effects and potential mechanism of Foshou Powder on Parkinson’s disease. Methods Reserpine was injected in KM mice to establish Parkinson’s disease model, and hypothermia and akinesia were measured to evaluate the effects of volatile oil of Angelica sinensis, Ligusticum chuanxiong and combination. The human monoamine oxidase(h MAO) activity and inhibition mode were measured by fluorescence spectrophotometry in vitro with kynuramine as the common substrate of human monoamine oxidase A(hMAO-A) and human monoamine oxidase B(hMAO-B). On this basis, the 2 × 4 factorial design was applied to research the interaction between Angelica sinensis volatile oil and Ligusticum chuanxiong volatile oil. Results The antagonistic experiments of reserpine showed that the application of Angelica sinensis volatile oil and Ligusticum chuanxiong volatile oil significantly improved hypothermia and akinesia. Enzyme activity test revealed that volatile oil of Angelica sinensis and Ligusticum chuanxiong had competitive inhibitory effects on hMAO-A and hMAO-B, and the combination of the two substances had significant synergistic effect on the activity of both hMAO. Conclusion Angelica sinensis and Ligusticum chuanxiong in Foshou Powder have significant synergistic effects in the prevention and treatment of Parkinson’s disease, which may be related to the inhibition of MAO activity.

关 键 词：佛手散 当归挥发油 川芎挥发油 人单胺氧化酶 帕金森病 析因设计 

分 类 号：R285.5[医药卫生—中药学]