基于LC-MS代谢组学技术的注射用黄芪多糖活性成分调控巨噬细胞代谢研究  

Study on active constituents of Astragalus polysaccharides for injection to regulate metabolism of macrophages based on LC-MS metabolomics

作　　者：李树颖 李科[1,4] 秦雪梅 李先荣[3] 杜昱光[4] LI Shu-ying;LI Ke;QIN Xue-mei;LI Xian-rong;DU Yu-guang(Modern Research Center for Traditional Chinese Medicine,Shanxi University,Taiyuan 030006,China;College of Chemistry and Chemical Engineering,Shanxi University,Taiyuan 030006,China;Shanxi Academy of Traditional Chinese Medicine,Taiyuan 030006,China;Institute of Process Engineering,Chinese Academy of Sciences,Beijing 100190,China)

机构地区：[1]山西大学中医药现代研究中心,山西太原030006 [2]山西大学化学化工学院,山西太原030006 [3]山西省中医药研究院,山西太原030006 [4]中国科学院过程工程研究所,北京100190

出　　处：《中草药》2020年第6期1575-1585,共11页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金资助项目(81872962);山西省优秀人才科技创新项目(201605D211030);山西省优秀人才科技创新项目(201705D211020);山西省重点研发计划重点项目(201603D311101);国家中药标准化项目(ZYBZH-Y-JIN-34);山西省科技攻关项目(20150313004-5)。

摘　　要：目的采用LC-MS代谢组学方法,研究注射用黄芪多糖活性成分对小鼠单核巨噬细胞白血病细胞Raw264.7吞噬活性的影响。方法采用中性红法检测注射用黄芪多糖中不同相对分子质量多糖对Raw 264.7细胞吞噬活性的影响,筛选出活性成分,并对细胞培养液和细胞裂解液进行LC-MS分析,结合多元统计分析和代谢通路分析,探索其作用机制。结果注射用黄芪多糖的小相对分子质量多糖在质量浓度为30μg/m L时能显著增强Raw 264.7细胞的吞噬活性。与对照组比较,注射用黄芪多糖活性成分作用于Raw264.7细胞后,细胞内外共发现41种差异代谢物,主要涉及氨基酸代谢、能量代谢及抗氧化作用。结论注射用黄芪多糖的小相对分子质量多糖能提高巨噬细胞吞噬活性,其机制可能与氨基酸代谢、能量代谢及抗氧化作用密切相关。Objective To study the phagocytosis of mouse monocyte-macrophage Raw 264.7 by the active constituents of Astragalus polysaccharides for injection based on LC-MS metabolomics. Methods Neutral red method was used to detect the phagocytosis of Raw 264.7 by different molecular weight fractions of Astragalus polysaccharides for injection. The active components were selected, and the cell culture supernatant and cell lysate were analyzed by LC-MS. Combined with multivariate statistical analysis and metabolism pathway analysis, the mechanism of action was studied. Results The small molecular weight fraction of Astragalus polysaccharides for injection significantly promoted the phagocytosis of Raw 264.7 at a concentration of 30 μg/m L. Compared with the control group, after the active part of the injection of Astragalus polysaccharides was applied to Raw 264.7, 41 differential metabolites were found in and out of the cells, mainly related to amino acid metabolism, energy metabolism and antioxidant effects. Conclusion The small molecular weight fraction of Astragalus polysaccharides for injection can increase the phagocytosis of Raw 264.7, and its mechanism may be closely related to amino acid metabolism, energy metabolism and antioxidant effects.

关 键 词：注射用黄芪多糖 吞噬 代谢组学 氨基酸代谢 能量代谢 抗氧化 

分 类 号：R285.5[医药卫生—中药学]