不同产地野生远志中5种主要成分的含量测定及质量分析  

Content determination and quality analysis of five main components in Polygala tenuifolia from different habitats

作　　者：张琳 杨冰月 王晶 张岗 颜永刚 高静 杨新杰 胡本祥 彭亮 ZHANG Lin;YANG Bing-yue;WANG Jing;ZHANG Gang;YAN Yong-gang;GAO Jing;YANG Xin-jie;HU Ben-xiang;PENG Liang(Shaanxi Qinling Application Development and Engineering Center of Chinese Herbal Medicine,College of Pharmacy,Shaanxi University of Chinese Medicine,Xi’an 712046,China)

机构地区：[1]陕西中医药大学药学院陕西省秦岭中草药应用开发工程技术研究中心,陕西西安712046

出　　处：《中草药》2020年第6期1609-1616,共8页Chinese Traditional and Herbal Drugs

基　　金：公益性行业(中医药)科研专项经费项目(201507002-1-08);陕西中医药大学“秦药”品质评价及资源开发学科创新团队项目(2019-QN01);2019年医疗服务与保障能力提升补助资金(中医药事业传承与发展部分)“全国中药资源普查项目”(财社[2019]39号);2018全国中药特色技术传承人才(20184828005)。

摘　　要：目的研究不同产地野生远志Polygala tenuifolia中5种主要成分的含量差异,为远志多成分质量评价体系提供基础。方法采用HPLC法测定20个产地野生远志中远志酮Ⅲ、3,6′-二芥子酰基蔗糖、远志酸、远志皂苷元和细叶远志皂苷的含量,运用SPSS 20.0和SIMCA 11.5进行差异性、聚类、相关性和主成分分析。结果不同产地野生远志中5种主要成分的含量差异显著,聚类分析和主成分分析可将所有样品聚为2类,第Ⅰ类所含3,6′-二芥子酰基蔗糖、远志酮Ⅲ、远志皂苷元和细叶远志皂苷含量较高,来自陕西渭南、陕西咸阳和山西新绛,其余样品则聚为第Ⅱ类。结论陕西渭南、陕西咸阳和山西新绛所产野生远志中主要成分含量较高,与其他产地存在极显著性差异,可为远志质量评价、优良种质及栽培基地选择等提供依据。Objective The contents of five main components in Polygala tenuifolia from different habitats were determined, which provided certain data support and theoretical basis for the quality evaluation system of P. tenuifolia. Methods The contents of polygalaxanthone Ⅲ, 3,6′-disinapoyl sucrose, polygalacic acid, senegenin and tenuifolin of roots from different wild samples were determined by HPLC. Then SPSS 20.0 and SIMCA 11.5 were used for difference analysis, correlation analysis, hierarchical cluster analysis(HCA), and principal component analysis(PCA). Results The content of the five main components in wild P. tenuifolia samples from different habitats was significantly different. The 20 samples were placed into two clusters(Ⅰ, Ⅱ) by HCA and PCA. Cluster Ⅰ comprised three samples with higher content of 3,6′-disinapoyl sucrose, polygalaxanthone Ⅲ, senegenin and tenuifolin from Weinan, Xianyang in Shannxi Province, and Xinjiang in Shanxi Province, whereas cluster Ⅱ contained the other 17 samples. Conclusion The results showed that the main components of P. enuifolia from Weinan, Xianyang in Shannxi Province and Xinjiang in Shanxi Province were significantly higher than other origins, and which provided a reference for the quality control, selection of excellent germplasm and cultivation bases of P. tenuifolia.

关 键 词：远志 质量评价 远志酮Ⅲ 3 6′-二芥子酰基蔗糖 远志酸 远志皂苷元 细叶远志皂苷 

分 类 号：R286.2[医药卫生—中药学]