液相色谱-串联质谱法筛查鉴定203种毒品  

Screening and Identifying of 203 Drugs by Liquid Chromatography-tandem Mass Spectrometry

作　　者：张宇笑 盛振海 梁晨[2] 汪蓉[2] 蔡正艳[1] 张玉荣[2] ZHANG Yuxiao;SHENG Zhenhai;LIANG Chen;WANG Rong;CAI Zhengyan;ZHANG Yurong(Shanghai Institute of Pharmaceutical Industry,China State Institute of Pharmaceutical Industry,State Key Laboratory of Innovative Pharmaceuticals and Pharmaceutical Industry,Shanghai 201203,China;Institute of Forensic Evidence,Shanghai Key Laboratory of Scene Evidence,Shanghai Municipal Public Security Bureau,Shanghai 200083,China)

机构地区：[1]中国医药工业研究总院上海医药工业研究院创新药物与制药工艺国家重点实验室,上海201203 [2]上海市公安局物证鉴定中心上海市现场物证重点实验室,上海200083

出　　处：《中国司法鉴定》2020年第2期25-36,共12页Chinese Journal of Forensic Sciences

基　　金：上海市科委科研计划项目(16dz1205600)。

摘　　要：目的建立203种毒品的液相色谱-串联质谱筛查鉴定方法。方法选用Accucore TM Phenyl/Hexyl苯基己基柱(100 mm×2.1 mm,2.6μm)为色谱柱,柱温50℃,以甲醇乙腈混合溶剂(体积比1:1,含0.1%甲酸和2 mmol/L甲酸铵)、水(含0.1%甲酸和2 mmol/L甲酸铵)作为流动相进行梯度洗脱,流速0.4 mL/min。质谱采用电喷雾正离子模式(ESI+)进行离子化,使用多反应监测(MRM)模式采集数据,总分析时间14 min。结果该方法实现了203种毒品的筛查鉴定分析,各目标物的方法检出限均为10 ng/mL。结论建立的筛查鉴定方法具有快速、准确、灵敏等优点,能够满足禁毒工作的日常需要。Objective A method using liquid chromatography-tandem mass spectrometry(LC-MS/MS)was established for screening and identifying of 203 drugs simultaneously.Methods An AccucoreTM Phenyl/Hexyl column(100 mm×2.1 mm,2.6μm)was selected as the column.The column temperature was 50℃.The samples were dissolved and diluted with methanol.The analytes were separated by gradient elution at a flow rate of 0.4 mL/min using the mobile phase of 0.1%(v/v)formic acid and 2 mmol/L ammonium formate both in 50%(v/v)methanol-acetonitrile mixed solution and water.The analytes were detected by electrospray ionization in positive mode with the multiple-reaction monitoring(MRM)scan mode,and the total running time was 14 minutes.Results The method realized the screening and identifying of 203 drugs.Method detection limit of each analyte was 10 ng/mL.Conclusion This developed method was rapid,accurate,sensitive.It is gualified for the daily application of drug controluse.

关 键 词：液相色谱-串联质谱 毒品筛查 司法鉴定 

分 类 号：DF795.1[医药卫生—法医学]