大黄-积雪草不同比例配伍对大黄游离蒽醌溶出率的影响  

Effect of Different Proportion Compatibility of Rhei Rhizoma et Radix-Centellae Herba on Dissolution Rate of Rree Anthraquinone

作　　者：韩怡[1] 柳春娣 HAN Yi;LIU Chun-di(Pharmacy Department of Zhangjiagang Hospital Affiliated to Nanjing University of TCM,Zhangjiagang 215600,China)

机构地区：[1]南京中医药大学附属张家港医院药学部,江苏张家港215600

出　　处：《中国现代中药》2020年第1期94-97,共4页Modern Chinese Medicine

基　　金：江苏省苏州市产业技术创新专项项目(SYSD2016161)。

摘　　要：目的:研究大黄-积雪草不同比例配伍对大黄5个游离蒽醌(芦荟大黄素、大黄酸、大黄素、大黄酚、大黄素甲醚)溶出率的影响。方法:采用高效液相色谱法分析大黄单提液及大黄-积雪草不同比例配伍混提液中5个游离蒽醌的含量。色谱条件:Thermo Hypersil BDS C18色谱柱(250 mm×4.6 mm,5μm);流动相甲醇-0.1%磷酸溶液(85∶15),流速1 mL·min^-1,柱温30℃,检测波长254 nm。结果:6种比例大黄-积雪草混提液游离蒽醌含量均小于大黄单提液,积雪草对大黄5个游离蒽醌的溶出影响明显。结论:综合考虑大黄单提液及各配伍比例游离蒽醌含量,认为大黄-积雪草临床最佳配伍比例为1∶5。Objective:To study the effects of different proportions of Rhei Rhizoma et Radix and Centellae Herba on the dissolution rate of 5 free anthraquinone(aloe-emodin,rhein,emodin,chrysophanol,physcion).Methods:The content of 5 free anthraquinones in Rhei Rhizoma et Radix extract and Rhei Rhizoma et Radix and Centellae Herba were determined by HPLC.The tests were carried out by Thermo C18(250 mm×4.6 mm,5μm)by isocratic elution with methanol and 0.1%phosphoric acid water solution as mobile phase at a flow rate of 1 mL·min^-1,the column temperature was 30℃and the detection wavelength was 254 nm.Results:The content of free anthraquinone in 6 kinds of Rhei Rhizoma et Radix and Centellae Herba mixture was lower than Rhei Rhizoma et Radix extract.The effect of Centellae Herba on the dissolution of 5 free anthraquinones from Rhubarb was obvious.Conclusion:The best compatibility ratio of Rhei Rhizoma et Radix and Centellae Herba is 1∶5 by comprehensive consideration of the content of free anthraquinone in Rhubarb single extract and each compatibility ratio.

关 键 词：大黄-积雪草 配伍比例 游离蒽醌 高效液相色谱法 

分 类 号：R282.71[医药卫生—中药学]