包膜颗粒的表面磷脂重排对细胞内吞的影响  

Effects oflipid redistribution on protocell nanoparticles uptake by cells

作　　者：王颖 王平 张国梁 朱涛[1] 蒋中英[1,2] WANG Ying;WANG Ping;ZHANG Guo-Liang;ZHU Tao;JIANG Zhong-Ying(National Laboratory of Solid State Microstructures,School of Physics,Nanjing University,Nanjing 210093,China;Key Laboratory of Micro-nano Electric Sensing Technology and Bionic Devices,College of Electronic and Information Engineering,Yili Normal University,Yining 835000,China)

机构地区：[1]南京大学,物理学院,固体微结构物理国家重点实验室,南京210093 [2]伊犁师范大学,电子与信息工程学院,微纳电传感技术与仿生器械重点实验室,伊宁835000

出　　处：《原子与分子物理学报》2020年第1期1-6,共6页Journal of Atomic and Molecular Physics

基　　金：国家自然科学基金地区项目(21764015);国家自然科学基金应急管理项目(11847610);国家自然科学基金青年项目(11904167);伊犁师范大学重点实验室开放课题基金项目(2016WNZD01,2016WNZD02)。

摘　　要：在纳米颗粒表面包裹生物膜可以增强体系的生物相容性、靶向性、内含物释放的可控性,但包膜颗粒与细胞膜作用的机制仍不清楚.在本研究中,我们考察了不同侧向流动性的负电性磷脂膜包裹的多孔硅纳米颗粒的体外细胞内吞行为.发现,高流动的液态磷脂包被产生了较高的内吞效率,并且它的内吞方式也与低流动的凝胶态磷脂包被情况存在差异.Derjaguin-Landau-Verway-Overbeek理论分析表明,前者的磷脂空间重排能够促进生物膜与细胞膜的融合与粒子内吞,而后者在膜融合过程中存在高能量势垒,因此只能以胞饮的方式被动地进入细胞.我们的研究深化了包膜粒子内吞过程的认识,为后续设计复杂的纳米载药体提供了新的思路和参考.Coating of biomembrane on the nanoparticles can enhance their biocompatibility,targeting and controllability of inclusions release.However,the interaction mechanism between the protocell nanoparticles and cell membranes remains unclear.In this study,we have investigated the in vitro endocytosis of porous silicon nanoparticles coated with negative lipid membranes with different lateral fluidities.It was found that the fluid liquid-phase lipid coating produced a high endocytosis efficiency,while its endocytosis pathway was also different from that of the low fluid gel-phase lipid coating.Derjaguin-Landau-Verway-Overbeek theoretical analysis showed that lipid rearrangement can promote the fusion between biomembrane and cell membrane as well as particle endocytosis in the former case.However,a high energy barrier was observed in the membrane fusion in the latter case.So a pinocytosis uptake pathway was preferred.Our research deepens the understanding of the endocytosis of protocell nanoparticles.It also provides new insight and references for the design of complex nanoparticle-based drug carriers in the future.

关 键 词：包膜颗粒 细胞内吞 DLVO理论 膜融合 胞饮 

分 类 号：O469[理学—凝聚态物理]