灵芝三萜类化合物对砷中毒大鼠脑组织氧化应激损伤的影响  

Effects of Ganoderma lucidum Triterpenoids on Oxidative Stress Injury in Brain Tissues of Rats with Arsenism

作　　者：张玥[1,2] 陈林 谢春[4] 杨小雪 罗俊[2] 罗喜荣 ZHANG Yue;CHEN Lin;XIE Chun;YANG Xiaoxue;LUO Jun;LUO Xirong(High Efficacy Application of Natural Medicinal Resources Engineering Center of Guizhou&Key Laboratory of Optimal Utilization of Natural Medicine Resources,Guizhou Medical University,Guiyang 550025,Guizhou,China;Department of Pharmacology,School of Basic Medical Sciences,Guizhou Medical University,Guiyang 550025,Guizhou,China;Department of Pharmacology,Qiannan Medical College For Nationalities,Duyun 558013,Guizhou,China;Department of Occupational Health and Environmental Hygiene,School of Public Health,Guizhou Medical University,Guiyang 550025,Guizhou,China;Center for Disease Control and Prevention in Southeast Guizhou,Kaili,556000,Guizhou,China;School of Pharmaceutical Sciences,Guizhou Medical University,Guiyang 550025,Guizhou,China)

机构地区：[1]贵州医科大学贵州省特色天然药物资源高效利用工程研究中心&天然药物资源优效利用重点实验室,贵州贵阳550025 [2]贵州医科大学基础医学院药理学教研室,贵州贵阳550025 [3]黔南民族医学高等专科学校药理学教研室,贵州都匀558013 [4]贵州医科大学公共卫生学院职业卫生与环境卫生学系,贵州贵阳550025 [5]黔东南州疾病预防控制中心,贵州凯里556000 [6]贵州医科大学药学院,贵州贵阳550025

出　　处：《贵州医科大学学报》2020年第3期277-280,共4页Journal of Guizhou Medical University

基　　金：贵州省科学技术基金[黔科合J字(2012)2028]。

摘　　要：目的:观察灵芝三萜类化合物(GLT)对砷中毒大鼠脑组织总抗氧化能力(T-AOC)、超氧化物歧化酶(SOD)活性及丙二醛(MDA)含量的影响。方法:60只大鼠随机均分为正常对照组(NS 10 m L/kg)、模型组(亚砷酸钠10 mg/kg)和GLT低、中、高剂量组(GLT 0.25、0.5、1.0 g/kg),连续砷染毒90 d后建立砷中毒大鼠模型;观察GLT对砷中毒大鼠脑组织中T-AOC、SOD活性及MDA含量的变化。结果:与正常对照组比较,模型组T-AOC和SOD活力明显降低,MDA含量明显升高(P<0.05);给予GLT干预后,与模型组比较,GLT各剂量组T-AOC、SOD活性明显上升(P<0.05),GLT中、高剂量组MDA含量明显降低(P<0.05)。结论:GLT能够改善砷中毒大鼠的氧化应激损伤,其作用机制可能与升高脑组织中T-AOC、SOD活性及降低MDA含量有关。Objective:To evaluate anti-oxidative effects of Ganoderma lucidum triterpenes(GLT)on arsenism-induced injury in the brain of rats by detecting the total antioxidant capacity(T-AOC)and superoxide dismutase(SOD)activity and malonaldehyde(MDA)content.Methods:60 rats were randomly divided into 5 groups:the control group(NS 10 mL·kg-1),the model group(sodium arsenite 10 mg·kg-1),GLT low,middle and high dose groups(GLT 0.25,0.5,1.0 g·kg-1).90 days after exposure to arsenic,the rat model of arsenic poisoning was established,and the indexes were detected by kit instructions to observe the changes of T-AOC,SOD activity and MDA content in the brain tissues of arsenic poisoning rats induced by GLT.Results:Compared with the control group,the activities of T-AOC and SOD in the model group decreased significantly,while the content of MDA increased significantly.After the intervention of GLT,compared with the model group,the activities of T-AOC and SOD increased significantly,while the content of MDA decreased significantly.Conclusion:GLT can improve the oxidative stress injury in rats with arsenism,and its mechanism may be related to the increase of T-AOC and SOD activity and the decrease of MDA content in brain tissue.

关 键 词：灵芝属 砷中毒 丙二醛 超氧化物歧化酶 灵芝三萜类化合物 总抗氧化能力 

分 类 号：R932[医药卫生—生药学] R992[医药卫生—药学]