贵州产灯盏细辛组织培养快速繁殖体系的建立  

Establishment of Rapid Propagation System to Culture Herbal Medicine Erigeron breviscapus in Guizhou Province

作　　者：张洁[1,2] 毛春燕 刘利利 曾丽娜 刘红美 ZHANG Jie;MAO Chunyan;LIU Lili;ZENG Lina;LIU Hongmei(Center of Medical Biotechnology Engineering Research&Key Laboratory of Biology and Medical Engineering,Guizhou Medical University,Guiyang 550025,Guizhou,China;Department of Biotechnology,College of Biology and Engineering,Guizhou Medical University,Guiyang,550025,Guizhou,China)

机构地区：[1]贵州医科大学医药生物技术工程研究中心&生物与医学工程重点实验室,贵州贵阳550025 [2]贵州医科大学生物与工程学院生物技术教研室,贵州贵阳550025

出　　处：《贵州医科大学学报》2020年第3期310-314,320,共6页Journal of Guizhou Medical University

基　　金：贵州医科大学医药生物技术工程研究中心(校工程中心2016002);贵州省教育厅项目[黔教合KY字(2018)088];贵州医科大学专创融合课程[校办发(2019)17-5]。

摘　　要：目的:探讨贵州灯盏细辛组织培养快速繁殖体系的建立。方法:贵州灯盏细辛种子为外植体获得无菌苗后,以无菌苗的叶片和叶柄作为外植体,添加不同浓度6-苄基腺嘌呤(6-BA)、奈乙酸(NAA)于Murashige&Skoog(MS)培养基中,筛选出最适愈伤组织诱导和芽分化增殖的培养基;再采用添加不同浓度的奈乙酸(NAA)和吲哚丁酸(IBA)于MS或1/2 MS中,筛选出最适生根培养基。结果:叶片不适合作为诱导愈伤的外植体,最适宜叶柄愈伤组织诱导的培养基为MS+1.0 mg/L 6-BA+0.3 mg/L NAA;最适宜不定芽分化增殖的培养基为MS+2.0 mg/L 6-BA+0.3 mg/L NAA,增殖倍数可达到12;最适宜生根的培养基为1/2MS+0.5 mg/L NAA+0.8 mg/L IBA,生根率可达100%。结论:本研究建立了贵州灯盏细辛的组织培养快速繁殖体系,为规模化生产奠定基础。Objective:To establish a rapid propagation system for Erigeron breviscapus tissue culture in Guizhou.Methods:The seeds of E.breviscapus were cultured to get sterile seedlings.The leaves and petioles of sterile seeding were sliced and added into Murashige&Skoog(MS)medium.6-benzyladenine(6-BA)andα-naphthalene acetic acid(NAA)at different concentration were added to MS medium to optimize the most sutiable medium for callus induction,bud proliferation.In addition,α-naphthalene acetic acid(NAA)and indole-3-butyric acid(IBA)at different concentration of were added to MS or 1/2 MS medium to screen the optimal medium for rooting.Results:The leaves were not suitable as callus-induced explants.The most optimal media were MS+1.0 mg/L 6-BA+0.3 mg/L NAA for petiole callus induction,MS+2.0 mg/L 6-BA+0.3 mg/L NAA for adventitious bud differentiation(the multiplication factor up to 12),1/2 MS+0.5 mg/L NAA+0.8 mg/L IBA for rooting(the rooting rate up to 100%).Conclusion:This study establishes a rapid tissue culture system for E.breviscapus and provids a foundation for large-scale production.

关 键 词：组织培养技术 灯盏细辛 快速繁殖体系 激素配比 愈伤诱导 分化增殖 生根 

分 类 号：R282.2[医药卫生—中药学]