三七黑斑病菌对烯唑醇的敏感性研究  

Sensitivity of Alternaria of panax notoginseng to Diniconazole

作　　者：汤红 张鸿 张定平 龙月娟 毛忠顺[3] TANG Hong;ZHANG Hong;ZHANG Ding-ping;LONG Yue-juan;MAO Zhong-shun(School of Environment and Resources,Wenshan University,Wenshan 663099,China;School of Sanqi,Wenshan University,Wenshan 663099,China;Sanqi Inistitute of Science and Technology,Wenshan University,Wenshan 663099,China)

机构地区：[1]文山学院环境与资源学院,云南文山663099 [2]文山学院三七学院,云南文山663099 [3]文山学院三七研究院,云南文山663099

出　　处：《特产研究》2020年第2期32-36,共5页Special Wild Economic Animal and Plant Research

基　　金：云南省重大科技计划(2016ZF001);云南省教育厅科学研究基金项目(2018JS500);2017年地方高校国家级大学生创新训练项目(201711556001)。

摘　　要：为了解三七黑斑病菌对烯唑醇的敏感性,采用菌丝生长速率法测定了分离自云南省砚山、石林、丘北和西双版纳不同地区的50个三七黑斑病菌菌株对烯唑醇的敏感性。结果表明,50个供试菌株对烯唑醇的EC50值在0.1739~6.6707 g/mL,平均EC50值为(1.7845±1.1954)g/mL,已出现对烯唑醇敏感性降低的亚群体,其中41个菌株对烯唑醇的敏感性频率分布呈正态分布,将这41个菌株的EC50平均值(1.3542±0.6529)g/mL作为三七黑斑病菌对烯唑醇的相对敏感基线;其他9个菌株中,抗性菌株有2个,抗性指数在3~10。由此推测,供试的三七黑斑病菌已出现对烯唑醇产生抗药性的趋势。本研究为三七黑斑病菌抗药性风险评估提供了一定的理论依据。To evaluate the susceptibility of panax notoginseng to diniconazole,50 samples of Alternaria panax isolates from different regions of Yanshan,Shilin,Qiubei and Xishuangbanna in Yunnan Province were detected by mycelial growth rate method.The results showed that the EC50 values of the 50 test strains ranged from 0.1739 to 6.6707 g/mL,and the average EC50 value was(1.7845±1.1954)g/mL.Subpopulations with reduced sensitivity to diniconazole had been observed.41 strains had normal distributions,therefore,the average EC50 value of 41 strains(1.3542±0.6529)g/mLwas used as the relative baseline.Among the other 9 strains,there were two resistant strains,and the resistance index was 3-10.It was inferred that the Alternaria panax already had tendency of resistance to diniconazole.This study could provide theoretical basis for the risk assessment of resistance of Alternaria panax to fungicides.

关 键 词：三七 黑斑病菌 烯唑醇 敏感性 

分 类 号：S567.236[农业科学—中草药栽培]