淫羊藿叶HPLC指纹图谱的建立及2个化学成分含量的测定  

Establishment of HPLC Fingerprint of Epimedii Leaves and Determination of Two Chemical Components

作　　者：曹菁 王淑敏[1] 宋凤瑞[2,3] 皮子凤 CAO Jing;WANG Shu-min;SONG Feng-rui;PI Zi-feng(School of Pharmaceutical,Changchun University of Chinese Medicine Changchun 130117,China;Changchun Institute of Applied Chemistry Chinese Academy of Sciences,Changchun 130022,China;Jilin Key Laboratory of Traditional Chinese Medicine Chemistry and Mass Spectrometry,Changchun 130022,China)

机构地区：[1]长春中医药大学药学院,吉林长春130117 [2]中国科学院长春应用化学研究所,吉林长春130022 [3]中药化学与质谱吉林省重点实验室,吉林长春130022

出　　处：《特产研究》2020年第2期47-55,共9页Special Wild Economic Animal and Plant Research

基　　金：吉林省科技发展计划项目(20180201081YY);长春市科技局项目(18YJ008)。

摘　　要：建立15批次淫羊藿叶HPLC指纹图谱,测定淫羊藿叶中淫羊藿苷和淫羊藿次苷I的含量。运用高效液相色谱法建立淫羊藿叶指纹图谱并测定淫羊藿苷和淫羊藿次苷I的含量,其色谱条件为色谱柱:YMC-Pack ODS-A(4.6 mm 250 mm,5 m);流速:1.0mL/min;柱温:25℃;流动相:乙腈-0.1%甲酸水溶液梯度洗脱;检测波长:270 nm;运用中药色谱指纹图谱相似度评价系统(2012版)和IBM SPSS Statistics软件(20.0版)分别进行15批次淫羊藿叶指纹图谱相似度评价和聚类分析。结果表明,淫羊藿叶样品中淫羊藿苷和淫羊藿次苷I的含量分别为0.0135%~0.0249%和0.0024%~0.0034%;所创立的淫羊藿叶指纹图谱共有8个色谱峰;15批次淫羊藿叶指纹图谱整体相似度大于0.9,具有良好的相似度;聚类分析结果显示,15批次淫羊藿叶共分为4类。所建立的淫羊藿叶指纹图谱较好地反映了淫羊藿叶多组分的特征,所选淫羊藿药材在产地和批次上具有代表性,为后续淫羊藿药材的研究提供了参考。This study establish HPLC fingerprint of 15 batches of epimedii leaves and determine the components of icariin and icarisid I in the leave.Using HPLC method to establish the fingerprint and determine the contents of icariin and icarisid I,whose conditions are:chromatographic column:YMC-Pack ODS-A(4.6 mm 250 mm,5 m);column temperature:25℃;mobile phase:acetonitrile-0.1%formic acid;flow rate:1.0 m L/min;detection wavelength:270 nm;Using similarity evaluation for fingerprint of TCM(version 2012)and the software IBM SPSS Statistics(version 20.0)to carry out similarity evaluation and cluster analysis of 15 batches of epimedii leaves.The results show that the components of icariin and icarisid I in epimedii leave samples are 0.0135%~0.0249%and 0.0224%~0.0034%;the esablished fingerprint of epimedii leaves has 8 mutual fingerprint peaks in total;the whole similarities of the fingerprint of 15 batches of epimedii leaves are more than 0.9,having a good similarity;Cluster analysis shows the 15 batches of epimedii leaves are divided into four categories.The established fingerprint of epimedii leaves reflects the multi-component appearance.The choosen of epimedii leaves are representative in places and batches,providing a reference for further study of epimedii leaves.

关 键 词：淫羊藿叶 HPLC指纹图谱 相似度 聚类分析 

分 类 号：R284.1[医药卫生—中药学]