不同电子载体对丙酮丁醇发酵的影响研究  

Effects of Different Electronic Carriers on Acetone-butanol-ethanol Fermentation by Clostridium acetobutylicum ART18

作　　者：罗壹艳 刘孟荧 方利敏 周智友 李汉广[1] LUO Yi-yan;LIU Meng-yin;FANG Li-min;ZHOU Zhi-you;LI Han-guang(Jiangxi Key Laboratory for Conservation and Utilization of Fungal Resources/Jiangxi Engineering Laboratory for the Development and Utilization of Agricultural Microbial Resources,College of Bioscience and Bioengineering,Jiangxi Agricultural University,Nanchang 330045,China)

机构地区：[1]江西农业大学生物科学与工程学院/江西农业微生物资源开发与利用工程实验室/江西省菌物资源保护与利用重点实验室,江西南昌330045

出　　处：《生物灾害科学》2020年第1期85-89,共5页Biological Disaster Science

基　　金：国家自然科学基金(21466014);江西省教育厅科学研究(GJJ160388);江西农业大学学生创新创业计划(201810410015);江西农业大学博士启动基金(9232305387)。

摘　　要：通过研究不同浓度的丁酸钠、乙酸钠、中性红及组合添加中性红与弱酸盐等电子载体对菌种ART18发酵木薯粉产丁醇的影响。结果表明,丁酸钠、乙酸钠以及中性红均可提高丁醇产量,其中中性红效果最为显著,当添加量为3.0 g/L时,丁醇产量可达到(11.3±0.23)g/L,较对照组相比提高了51.7%;当组合添加3.0 g/L中性红与3.0g/L丁酸钠时,丁醇和总溶剂产量进一步提升,分别达到了(12.2±0.25)g/L和(17.4±0.18)g/L,较对照组分别提高了64.9%和33.8%,与单独添加丁酸钠和中性红相比,丁醇产量分别提高了32.6%和8.0%。说明组合添加中性红与弱酸盐对丁醇发酵有一定促进作用。In order to improve the acetone-butanol-ethanol(ABE) fermentation performance,the effects of different concentrations of electronic carriers,such as odium butyrate,sodium acetate,neutral red,and the combination of neutral red and weak acid salts,on butanol production from fermented cassava powder by the Clostridium acetobutylicum ART18 were investigated. The results showed that sodium butyrate,sodium acetate and neutral red could increase butanol production,of which,relatively speaking,neutral red contributed significantly to the synthesis of butanol;when the addition of neutral red was 3.0 g/L,the butanol production reached(11.3±0.23) g/L,which was 51.7% higher than that of the control group;when 3.0 g/L neutral red and 3.0 g/L sodium butyrate were added in combination,the butanol and total solvent production were further increased,reaching(12.2±0.25) g/L and(17.4±0.18) g/L,respectively,and the butanol production and the total solvent production were 64.9% and 33.8% higher than that of the control. Meanwhile,the yield of butanol increased by 32.6% and 8.0%,higher than that of the addition of sodium butyrate and neutral red alone,respectively. It was showed that the combination of neutral red and weak acid salt could promote the fermentation of butanol.

关 键 词：丁醇 丙醇丁醇梭菌ART18 电子载体 

分 类 号：TQ223.124[化学工程—有机化工]