心衰康及其拆方不同干预周期对心衰大鼠肾脏皮髓质AVPR1a、AVPR2的影响  

Influence of Xinshuaikang and Its Disassembled Parts Effect on AVPR1a and AVPR2 of Heart Failure Rats in Different Phases

作　　者：王振涛[1] 柴松波[1] 吴鸿[1] 芮浩淼[1] 边汝涛 WANG Zhentao;CHAI Songbo;WU Hong;RUI Haomiao;BIAN Rutao(The Second Affiliated Hospital of Henan University of Chinese Medicine,Zhengzhou 450011,Henan,China;Henan University of Chinese Medicine,Zhengzhou 450000,Henan,China)

机构地区：[1]河南中医药大学第二附医院,河南郑州450011 [2]河南中医药大学,河南郑州450000

出　　处：《中华中医药学刊》2020年第2期1-4,共4页Chinese Archives of Traditional Chinese Medicine

基　　金：国家自然科学基金(81072797)。

摘　　要：目的观察心衰康及其拆方4周、8周疗程对充血性心力衰竭(CHF)大鼠肾脏皮髓质中精氨酸加压素受体1A(AVPR1a)、精氨酸加压素受体2(AVPR2)的影响。方法对大鼠行冠状动脉结扎术造成心梗后心衰模型,随机分为6组,分别为模型组、心衰康全方组、益气组、温阳组、活血组、利水组。连续灌药4周及8周后观察动物尿量,并采用实时定量PCR法检测心衰康及其拆方对心衰大鼠肾脏皮髓质中AVPR1a、AVPR2的影响。结果心衰康全方及其拆方中的温阳、活血、利水药物均能降低下丘脑精氨酸血管加压素(AVP)mRNA表达;心衰康全方及其拆方中的活血、利水药物能明显降低心衰大鼠肾脏皮质、髓质AVPR1a mRNA、AVPR2 mRNA的表达。且随着用药时间的延长,益气、温阳药物对上述指标的抑制作用逐渐增强。结论心衰康全方及其拆方中的活血、利水药物能够通过降低心衰大鼠肾脏皮质、髓质AVPR1a mRNA、AVPR2 mRNA的表达,从而产生明显的利尿作用,达到减轻心脏前负荷,改善心功能的作用,随着用药时间的延长,益气、温阳药物对上述指标的抑制作用逐渐增强。Objective To observe the effect of Xinshuaikang and its disassembled parts on AVPR1 a and AVPR2 of heart failure failure(CHF)ratsin 4 and 8 weeks.Methods Rats underwent coronary ligation model of heart failure after myocardial infarction caused by ultrasound evaluation of left ventricular ejection fraction were divided into six groups,namely the model group,Xinshuaikang group,benefiting Qi group,warming Yang group,activating blood circulation group,promoting urination group.The urination was observed after 4 and 8 weeks.The real-time quantitative PCR was used to detect the influence on AVPR1 aand AVPR2.Results Xinshuaikang and its disassembled parts(warming Yang,activating blodo circulation and promoting urination)can reducethe expression of AVPmRNA.The parts which can activate blood circulation and promote urination can obviously reduce the expressions of AVPR1 a mRNA and AVPR2 mRNA in cortex and medulla of kidney.And the inhibiting effect was increasing with the time prolonging.Conclusion Xinshuaikang and its disassembled partswhich can activate blood circulation and promote urination can improve water metabolism pathway by reducing expressions of AVPR1 a mRNA and AVPR2 mRNAto reduce the load on the heart and improve heart function.As the medication time increasing,the inhibiting effect on the above indexes is gradually increasing.

关 键 词：慢性心衰 心衰康 肾脏皮髓质 AVPR1a AVPR2 

分 类 号：R285.5[医药卫生—中药学]