基于内毒素特异质模型的何首乌毒性与炮制工艺研究  

Investigation of Polygonum Multiflorum Radix Processing Approaches Using a Lipopolysaccharide Model of Hepatotoxivity

作　　者：薛琪 翟文泽 任晶[1,2,3,4] 孙飞 王淑美 梁生旺[1,2,3,4] 陈超 XUE Qi;ZHAI Wenze;REN Jing;SUN Fei;WANG Shumei;LIANG Shengwang;CHEN Chao(Guangdong Pharmaceutical University,Guangzhou 510006,Guangdong,China;Key Laboratory of Digital Quality Evaluation of Chinese Materia Medica,Guangzhou 510006,Guangdong,China;Engineering&Technology Research Center for Chinese Materia Medica Quality of Universities of GuangdongProvince,Guangzhou 510006,Guangdong,China;Engineering&Technology Research Center for Chinese Materia Medica Quality of Guangdong Province,Guangzhou 510006,Guangdong,China)

机构地区：[1]广东药科大学,广东广州510006 [2]国家中医药管理局中药数字化质量评价技术重点研究室,广东广州510006 [3]广东高校中药质量工程技术研究中心,广东广州510006 [4]广东省中药质量工程技术研究中心,广东广州510006

出　　处：《中华中医药学刊》2020年第2期162-165,I0030-I0032,共7页Chinese Archives of Traditional Chinese Medicine

基　　金：科技部科技基础性工作专项(2014FY111100-6)。

摘　　要：目的基于内毒素特异质肝损伤模型,考察不同炮制程度的何首乌对大鼠肝脏的损伤作用,以优选何首乌最为安全的炮制工艺。方法采用无毒剂量的脂多糖(LPS,尾静脉注射2.8 mg/kg)制备内毒素特异质模型大鼠,模型大鼠和正常大鼠均灌胃给予12.96 g/kg不同炮制程度(0、8、16、24、32 h)的何首乌50%乙醇提取物,各给药1次,检测大鼠血浆中丙氨酸氨基转移酶(ALT)、天冬氨酸氨基转移酶(AST)水平以及HE染色观察肝脏病理学改变。结果在灌胃给予不同炮制程度的何首乌50%乙醇提取物后,正常SD大鼠上,未见明显肝损伤作用,炮制24 h,32 h组ALT,AST值低于正常组;在内毒素特异质模型上,生首乌的ALT,AST值显著升高,炮制8 h,炮制16 h的ALT,AST值均高于正常组。结论何首乌以常压炮制32 h最为安全。Objective The objective of this study was to investigate the effects of Polygonum multiflorum radix on lipopolysaccharide(LPS)-mediated liver injury in rats following different degrees of Polygonum multiflorum radix processingin order to optimize Polygonum multiflorum radix processing approaches.Methods Rats received a non-fatal dose of LPS via caudal vein injection,after having been intragastrically administered 12.96 g/kg of 50%ethanol extract of Polygonum multiflorum radix that had been processed for a range of times(0,8,16,24,32 h).After 7 h,plasma samples and liver samples were collected for measurement of serum ALT/AST and histopathological assessment,respectively.Results Normal rats exhibited no significant liver injury.When Polygonum multiflorum radix samples had been processed for 24 h or 32 h.The mean ALT and AST values were lower relative to untreated controls.When rats also received LPS model,ALT and AST levels associated with Polygonum multiflorum radix were significantly increased,with those values of rats receiving Polygonum multiflorum radix prepared for 8 h and 16 h being higher than those in the normal control group.Conclusion The safest approach to Polygonum multiflorum radix processing is a 32 h processing approach conducted under normal pressure.

关 键 词：内毒素特异质模型 何首乌 制何首乌 炮制工艺 

分 类 号：R283[医药卫生—中药学]