理化条件对人感染H9N2禽流感病毒灭活效果的评价  

Evaluation of the inactivation performance under physical and chemical conditions against human infected H9N2 avian influenza viruses

作　　者：甘芳容 张烨[1] 谭敏菊[1] 成艳辉[1] 薄洪[1] 房琼琼 周为民[1] 王大燕[1] 董婕[1] 武桂珍[1] Gan Fangrong;Zhang Ye;Tan Minju;Cheng Yanhui;Bo Hong;Fang Qiongqiong;Zhou Weimin;Wang Dayan;Dong Jie;Wu Guizhen(National Institute for Virus Disease Control and Prevention,Chinese Center for Disease Control and Prevention,Beijing102206,China)

机构地区：[1]中国疾病预防控制中心病毒病预防控制所,北京102206

出　　处：《中华实验和临床病毒学杂志》2020年第1期87-91,共5页Chinese Journal of Experimental and Clinical Virology

基　　金："十三五"国家科技重大专项课题(2018ZX10734-401)。

摘　　要：目的评价温度、紫外线以及3种消毒剂对人感染H9N2禽流感病毒的灭活效果,确定H9N2病毒的灭活条件。方法含有10^10.67TCID50/ml病毒悬液分别在50℃、56℃、60℃、65℃温度下作用10~60 min;紫外线(UV)照射从10 min至80 min,每间隔10 min取样。物理条件作用的病毒残留活性在MDCK细胞上测定,通过Reed-Muench方法计算组织半数感染剂量(TCID50)来评估物理灭活效果。含有1010.37EID50/ml病毒悬液与等体积的10%的84消毒液、75%乙醇、1%浓度Virkon溶液作用1~15 min后接种SPF鸡胚,通过病毒鸡胚培养是否阴性来评估杀灭效果。当病毒滴度下降4 lgTCID50/ml或鸡胚病毒培养阴性视为该方法有效。结果人感染H9N2禽流感病毒经56℃15 min处理后病毒滴度下降4.02 lg TCID50,而作用30 min后病毒活性将降低到检测水平以下;60℃和65℃热灭活大于10 min,病毒活性将降低到检测水平以下。在UV照射20 min后病毒活性下降5.67 lgTCID50,作用70 min后病毒将降低到检测水平以下。10%84消毒剂、75%乙醇消毒液、1%Virkon作用3 min之后不能检测到病毒增殖。结论人感染H9N2禽流感病毒需要在特定条件下才能被有效灭活,我们的研究为人感染H9N2禽流感病毒的生物安全操作提供了实验依据。Objective Assess and determine inactivation effect of heat,.ultraviolet(UV)light and three disinfectants against human infected H9N2 avian influenza virus in laboratory.Methods Suspension containing with 10^10.67 TCID50/ml viral was exposed to 50℃,56℃,60℃,65℃for 10 to 60 minutes and UV every 10 interval minutes from 10 to 80 minutes.The residual viruses after physical treatment were determined through half of tissue culture infective dose(TCID50)with MDCK cells and calculated by Reed-Muench method.Suspension with 1010.37EID50/ml quantitative virus was applied to equal volume of 10%84 sanitizer,75%ethanol,1%Virkon solution and incubated for 1 minute to 15 minutes respectively.The residual viral activity would be evaluated by inoculating in SPF chicken embryo.When the virus titer dropped by 4 lgTCID50/ml or virus in chicken embryo culture was observed to be negative,the physical and chemical treatment was considered effective.Results Human infected H9N2 avian influenza virus titer decreased by 4.02 lgTCID50 at 56℃for 15 minutes,and after 30 minutes at 56℃or 10 minutes at 60℃/65℃,the post-viral titer would decline below the detection level.20 minutes of UV irradiation would lead to a 5.67 log reduction,and after 70 minutes lighted,the virus titer fell below the detection level.Virus proliferation was not detected after 3 minutes of disinfection with 10%84 sanitizer,75%ethanol and 1%Virkon.Conclusions We should note that it is necessary to meet the specific condition to effectively inactivate the human infected H9N2 avian influenza virus.Our study provides an experimental basis for the biosafety operation of human infected H9N2 avian influenza virus.

关 键 词：人感染禽流感病毒 H9N2亚型 灭活评估 实验室生物安全 

分 类 号：R51[医药卫生—内科学]