成体神经干细胞诱导方案研究进展  

Advances in research on induction strategies of adult neural stem cells

作　　者：刘秉春[1] 袁建龙[1] 邱英 云升[1] Liu Bingchun;Yuan Jianlong;Qiu Ying;Yun Sheng(Stem Cell Research Centre,Affiliated Hospital of Inner Mongolia Medical University,Huhhot 010050,China)

机构地区：[1]内蒙古医科大学附属医院干细胞研究中心,呼和浩特010050

出　　处：《中华神经科杂志》2020年第3期217-220,共4页Chinese Journal of Neurology

基　　金：内蒙古自治区科技计划项目(201802150);内蒙古医科大学科技百万工程项目(YKD2016KJBW005)。

摘　　要：近年来,研究人员致力于构建遗传和表型稳定的神经干细胞系,旨在恢复神经组织不可逆转的功能丧失从而满足临床需求。其中,成人体内维持其多能状态的干细胞也成为研究的焦点之一。随着诱导多能干细胞和体细胞直接转分化为所需细胞类型等技术的发展,基于使用来自胚胎或胎儿神经组织的同种异体神经干细胞的研究方法逐渐成为过去。本文围绕诱导干细胞和重编程体细胞的多能状态维持及诱导神经干细胞的实验方案做一综述。For decades,researchers have focused on building genetically and phenotypically stable neural stem cell lines designed to restore the irreversible loss of function of nerve tissue to meet clinical needs.Among them,stem cells that maintain their pluripotent state in adults have also become one of the research focuses.With the development of technologies such as induced pluripotent stem cells and direct differentiation of somatic cells into desired cell types,research methods based on the use of allogeneic neural stem cells derived from embryonic or fetal neural tissue have gradually become a thing of the past.This article will review the basic molecular mechanisms surrounding the maintenance of pluripotent states of stem cells and reprogrammed somatic cells,as well as experimental protocols for inducing neural stem cells.

关 键 词：成体干细胞 多能干细胞 细胞培养技术 神经系统 

分 类 号：R3[医药卫生—基础医学]