野生大豆ZYD00006回交导入系构建  

Construction of wild soybean backcross introgression lines

作　　者：蒋洪蔚[1,2] 李灿东 李瑞超 李莹莹[1] 尹燕斌 马占洲 曾庆力 张闻博 刘春燕 陈庆山 JIANG Hong-wei;LI Can-dong;LI Rui-chao;LI Ying-ying;YIN Yan-bin;MA Zhan-zhou;ZENG Qing-li;ZHANG Wen-bo;LIU Chun-yan;CHEN Qing-shan(Northeast Agricultural University,Harbin 150030,China;Jilin Academy of Agricultural Sciences,Changchun 130000,China;Jiamusi Branch Academy of Heilongjiang Academy of Agricultural Sciences,Jiamusi 154007,China)

机构地区：[1]东北农业大学,黑龙江哈尔滨150030 [2]吉林省农业科学院,吉林长春130000 [3]黑龙江省农业科学院佳木斯分院,黑龙江佳木斯154007

出　　处：《中国油料作物学报》2020年第1期8-16,共9页Chinese Journal of Oil Crop Sciences

基　　金：吉林省农业科技创新项目(C92070420);国家自然科学基金(31771882);黑龙江省自然科学基金(C2018017)。

摘　　要：为挖掘野生大豆优异稀有基因,2006年至今以栽培大豆绥农14(轮回亲本)与野生大豆ZYD00006(供体亲本)为双亲材料,经杂交、回交,标记辅助选择构建获得一套覆盖野生大豆全基因组的染色体片段导入系(代换系)。该群体共192个株系,包含野生大豆目标导入片段237个,平均每个连锁群的导入片段个数为11.85个;导入片段总长度1865.17 cM,覆盖整个基因组的82.43%。其中L连锁群野生大豆基因组覆盖率最高,为100%,N连锁群覆盖最低为53.17%。最长导入片段43.30 cM,最短导入片段0.22 cM。高度一致的遗传背景对大豆重要基因及野生大豆特有优异基因挖掘具有重要意义。同时,野生资源的引入极大丰富了栽培大豆的遗传基础,进而使得导入系后代表型变异丰富,为大豆遗传育种提供重要的材料基础。To excavate the excellent rare gene of wild soybean,this study had been carried out since 2006,Sui nong 14(recurrent parent)and wild soybean ZYD00006(donor parent)were used as parents to construct a set of chromosome fragment introduction lines(substitution lines)covering the whole genome of wild soybean through hy bridization,backcross and marker assisted selection.A total of 192 plant rows in this population,included 237 wild soybean introduction fragments,with the average number of introduction fragments per linkage group of 11.85.The total length of the introduction fragment was 1865.17cM with the coverage of 83.23%of wild soybean genome.The coverage rate of linkage group L was the highest of 100%;linkage group N had the least coverage,of 53.17%.The maximum length of segment was 43.30 cM and the minimum one was 0.22 cM.Highly consistent genetic back ground was of great significance for excavating important genes of soybean and wild soybean-specific excellent gene.At the same time,the introduction of wild resources greatly enriched the genetic basis of cultivated soybean,and further enriched the phenotypic variation of the introgression lines,providing an important material basis for soybean genetic breeding.

关 键 词：野生大豆 全基因组 回交导入系 

分 类 号：S565.1[农业科学—作物学]