单碘乙酸诱导SD大鼠膝骨性关节炎模型在不同时间点与疾病进程的评价  

Evaluation of Monoiodoacetic Acid-induced Knee Osteoarthritis SD Rats with Diseasse Progression at Different Time Points

作　　者：肖昆林 张蕊[2] 孙红 肖昆太 马建兵 XIAO Kunlin;ZHANG Rui;Sun Hong;XIAO Kuntai;MA Jianbing(Huadu District People's Hospital of Guangzhou,Guangzhou 510800,China;Xi'an Honghui Hospital,Xi'an 710054,China;Xiamen University School of Medicine,Xiamen 361000,China)

机构地区：[1]广州市花都区人民医院,广州510800 [2]西安交通大学附属红会医院,西安710054 [3]厦门大学医学院,厦门361000

出　　处：《实验动物与比较医学》2020年第1期47-52,共6页Laboratory Animal and Comparative Medicine

基　　金：陕西省自然科学基金资助项目(2018JM7081);陕西省自然科学基金资助项目(2018JM7039);西安市卫生局科研项目(No.J2019020030)。

摘　　要：目的用单碘乙酸(MIA)诱导膝骨性关节炎(OA)大鼠模型,于不同时间点进行检测和评估,分析与临床OA发病进程的相似性.方法24只2月龄雄性SD大鼠,随机分为OA模型组(18只)和正常对照组(6只).适应性喂养3d后,模型组大鼠右膝关节腔内注射2 mg MIA(溶于50μL生理盐水),对照组大鼠右膝关节腔内注射等量的生理盐水,注射后1周、2周和4周分别处死模型组和对照组中的大鼠,收集各组大鼠膝关节软骨组织标本,分别进行形态学观察、组织学染色和OA软骨组织病理学评估系统(OOCHAS)评分.结果模型组大鼠在造模1周,2周和4周后出现关节软骨组织的损伤,软骨细胞数量减少,潮线模糊,甲苯胺蓝和番红O染色变淡,并呈进行性加重趋势;模型组大鼠OOCHAS大体评分和组织学评分随时间的延长显著升高,造模后1周和2周相当于疾病早中期,4周后相当于疾病晚期.结论通过关节腔内注射MIA成功诱导了膝OA大鼠模型,并将不同时间点的结果与临床疾病进程相联系,为后期功能和机制研究奠定了基础.Objective To evaluate disease progression of monoiodoacetic acid-induced knee osteoarthritis(OA)rat model at different time points.Methods Twenty-four male SD rats at the age of two month were randomly divided into model group(18 rats)and control group(6 rats).In the model group,2 mg monoiodoacetic acid(MIA)dissolve in 50 μL saline was injected into the right knee joint of the mice,and the control group mice were injected with the same amount of saline.After 1 week,2 weeks and 4 weeks respectively,6 rats in each group were sacrificed,and the joint specimens were collected for morphological observation and histological analysis(HE,toluidine blue,and Safranin O staining).OA cartilage histopathology assessment system(OOCHAS)scoring and histological scoring were performed to evaluate the disease progression.Results The model group showed defects in articular cartilage,reduced number of chondrocytes,blurred tide line and lightened toluidine blue and safranin O staining.The severity was aggravated after 1 weeks,2 weeks and 4 weeks.The OOCHAS gross score and histological score was significantly increased with time extended.Conclusion The rat model of knee OA was successfully induced by intra-articular injection of monoiodoacetic acid,and the results at different time points were correlated with the clinical disease process,which laid a fundament for later functional and mechanism experiments.

关 键 词：骨性关节炎(OA) 单碘乙酸(MIA) 动物模型 OA软骨组织病理学评估系统(OOCHAS) 

分 类 号：Q749.72[生物学—分子生物学] Q9533