临床中药饮片黄芩的质量评价  

Quality Evaluation of Radix Scutellariae in Clinical Chinese Medicine

作　　者：管仁伟[1,2] 郭瑞齐 马飞[3] 陈兵[2] 林慧彬[2] 林建强[4] GUAN Renwei;GUO Ruiqi;MA Fei;CHEN Bing;LIN huibin;LIN jianqiang(Shandong University of Traditional Chinese Medicine,Jinan 250355,Shandong,China;Shandong Academy of Chinese Medicine,Jinan 250014,Shandong,China;The Affiliated Hospital of Shandong University of TCM,Jinan 250011,Shandong,China;State Key Lab of Microbial Technology,Shandong University,Jinan 250100,Shandong,China)

机构地区：[1]山东中医药大学药学院,山东济南250355 [2]山东省中医药研究院,山东济南250014 [3]山东中医药大学附属医院,山东济南250011 [4]山东大学微生物技术国家重点实验室,山东济南250100

出　　处：《辽宁中医杂志》2020年第2期155-157,共3页Liaoning Journal of Traditional Chinese Medicine

基　　金：山东省自然科学基金(ZR2017MH023,ZR2018PH040,ZR2015PH009);国家重点研发计划(2017YFC1701500,2017YFC1701502,2017YFC1701504,2017YFC1702700,2017YFC1702701);国家标准化建设项目(ZYBZH-Y-SD-33);2017年中医药公共卫生服务补助专项“全国中药资源普查项目”(财社[2017]66号);2018国家中医药管理局全国中药资源普查项目(GZY-KJS-2018-004);山东省重点研发计划(2015GSF119011);泰山学者工程(ts201511107);山东省中医药科技发展计划(2017-519)。

摘　　要：目的建立同时测定黄芩饮片中黄芩苷、汉黄芩苷、黄芩素、汉黄芩素含量的方法,对不同产地规格临床中药饮片黄芩进行质量评价。方法运用高效液相色谱法,采用Thermo Acclaim120-C18(4. 6 mm×250 mm,5μm)色谱柱,以乙腈(A)、0. 2%磷酸溶液(B)为流动相梯度洗脱,流速为1. 0 m L/min,进样量为10μL,检测波长为280 nm,柱温为30℃。结果黄芩苷、汉黄芩苷、黄芩素、汉黄芩素的质量分别在0. 1500~1. 1998μg(r=0. 9994)、0. 0499~0. 3994μg(r=1. 0000)、0. 0025~0. 0202μg(r=0. 9989)、0. 0025~0. 0198μg(r=1. 0000)范围内具有良好的线性关系。20批样品中上述4种黄酮类成分的平均含量范围分别为60. 4~148. 58 mg/g、14. 77~30. 99 mg/g、0. 99~26. 82 mg/g、0. 74~6. 18mg/g。结论临床中药饮片黄芩质量状况差异很大,本实验建立并优化的黄芩饮片定量分析方法,可为不同规格黄芩饮片的质量评价及临床用药提供依据。Objective:To establish an HPLC method for simultaneous determination of baicalin,wogonoside,baicalein and wogonin in Scutellaria baicalensis slices and evaluate the quality of Scutellaria baicalensis slices with different origins and specifications.Methods:Using high performance liquid chromatography(HPLC),Thermo Acclaim120-C18(4.6 mm×250 mm,5μm)column was used for the gradient elution of acetonitrile(A)and 0.2%phosphoric acid solution(B).The flow rate was 1 mL/min,the sample volume was 10μL,the detection wavelength was 280 nm,and the column temperature was 30℃.Results:The quality of baicalin,wogonoside,baicalein and wogonin had good linear relationship in the range of 0.1500-1.1998μg(r=0.9994),0.0499-0.3994μg(r=1.0000),0.0025-0.0202μg(r=0.9989),0.0025-0.0198μg(r=1.0000).The average contents of the above four flavonoids in the 20 batch samples ranged from 60.4 mg/g to 148.58 mg/g,14.77 mg/g to 30.99 mg/g,0.99 mg/g to 26.82 mg/g,0.74 mg/g to 6.18 mg/g,respectively.Conclusion:The quality of Radix Scutellariae in clinical Chinese medicine varies greatly and the experiment established quantitative analysis method of Scutellaria baicalensis pieces and provided the basis for quality evaluation and clinical medication of Scutellaria baicalensis slices with different specifications.

关 键 词：黄芩 饮片 黄酮化合物 高效液相色谱法 临床使用 质量评价 

分 类 号：R286.0[医药卫生—中药学]