GSR基因多态性与噪声性听力损失易感性的关系  

Association between GSTP1 gene polymorphisms and susceptibility to noise-induced hearing loss

作　　者：袁璐璐 陈国顺 焦洁 周文慧 吴辉 谷桂珍 张焕玲 郑玉新[5] 余善法 Yuan Lulu;Chen Guoshun;Jiao jie;Zhou Wenhui;Wu Hui;Gu Guizhen;Zhang Huanling;Zheng Yuxin;Yu Shanfa(Department of Labor and Environment Health,College of Public Health,Zhengzhou University,Zhengzhou 450041,China;Henan Medical College,Zhengzhou 451191,China;Henan Provincial Institute for Occupational Health,Zhengzhou 450052,China;Wugang Institute for Occupational Health,Wugang 462599,China;College of Public Health,Qingdao University,Qingdao 266021,China)

机构地区：[1]郑州大学公共卫生学院劳动卫生与环境卫生学系,450041 [2]河南医学高等专科学校,郑州451191 [3]河南省职业病防治研究院,郑州450052 [4]舞钢劳动卫生职业病防治研究所,462599 [5]青岛大学公共卫生学院,266021

出　　处：《中华劳动卫生职业病杂志》2020年第2期101-107,共7页Chinese Journal of Industrial Hygiene and Occupational Diseases

基　　金：国家自然科学基金项目(81372940、81872574);国家科技支撑计划项目(2014BAI12B03)。

摘　　要：目的探讨谷胱甘肽还原酶(glutathione reductase,GSR)基因多态性与噪声性听力损失(noise-induced hearing loss,NIHL)易感性之间的关系.方法2019年7月采用1∶1巢式病例对照研究方法,以河南省某钢铁厂的6297名接触噪声作业工人为队列研究人群中,筛选接触噪声工龄≥3年、双耳高频(3000、4000、6000 Hz)平均听阈≥40 dB的研究对象选择为听力损失组;并按照同性别、同工种、年龄相差≤5岁,接触噪声工龄相差≤2年,听力测试中任一耳语频(500、1000、2000 Hz)的任一频段听阈≤25 dB的匹配标准选择为对照组,筛选出听力损失组和对照组各276人.对调查对象进行一般体格检查和问卷调查,进行纯音听力测试和作业场所噪声测量.采用中高通量单核苷酸多态性分型检测技术(SNPscanTM法)对研究对象的GSR基因的3个核苷酸位点的多态性进行检测,并采用条件logistic回归分析不同单核苷酸多态性(single nucleotide polymorphism,SNP)与NIHL的关系,及调整协变量后不同多态位点与NIHL发生风险的关系;以不同累积噪声暴露量(cumulative noise exposure,CNE)分层后,采用条件logistic回归分析不同位点与NIHL发生风险的关系.结果研究对象的年龄为(40.28±8.00)岁,接噪工龄为(18.71±8.92)年,研究对象接触噪声水平为80.05~93.35 dB(A),CNE为86.83~107.92 dB(A)·年.与对照组比较,听力损失组、CNE、饮酒和噪声接触水平差异均无统计学意义(P>0.05);而年龄、接噪工龄、吸烟、双耳高频平均听阈和双耳语频平均听阈水平有差异,差异有统计学意义(P<0.05).在调整吸烟、饮酒、高血压等因素后,在共显性模型下,与携带GG基因型比较,携带rs1002149 GT、rs2251780 GA基因型患NIHL的风险更高(OR=1.558,95%CI:1.028~2.361;OR=1.550,95%CI:1.020~2.355,P<0.05);与携带TT/GT基因型比较,携带rs1002149TT基因型患NIHL的风险更高(OR=1.494,95%CI:1.002~2.228,P<0.05);rs3779647位点基因型与患NIHL风险无关(P>0.05)Objective To investigate the association between the single nucleotide polymorphisms(SNPS)at rs1695 and rs6591256 in glutathione S-transferase P1(GSTP1)gene and susceptibility to noise-induced hearing loss in Chinese Han workers exposed to noise.Methods Using the 1:1 nested case-control study and taking 6297 workers exposed to noise in a steel plant in Henan province as the cohort study population in July 2019,we screened those who have been exposed to noise for≥3 years and whose binaural high frequency(3000,4000,6000 Hz)average hearing threshold is≥40 dB(A)into the case group.The control group was selected according to the matching criteria of the same sex,same type of work,and the age difference was not more than 5 years old,and the working age difference was not more than 2 years.276 subjects were selected into the case group and the control group respectively.The medium and high throughout single nucleotide polymorphism typing technology(SNPscanTM technology)was used to detect the polymorphism of three nucleotide sites of GSR gene,and conditional logistic regression was used to analyze the relationship between single nucleotide polymorphism(SNP)and NIHL,and the relationship between different polymorphic sites and the risk of NIHL after adjusting covariates.After stratification with different cumulative noise exposure(CNE),Conditional logistic regression analysis was used to analysis the risk of NIHL at different loci.Results The mean and standard deviation of age of the selected subjects was(40.28±8.00),the mean and standard deviation of noise-exposed working years was(18.7±8.92)years.The range of noise exposure levels and comulative noise exposure were 80.05-93.35dB(A)and 86.83-107.92 dB(A)·year,respectively.Compared with the control group,there were no statistically significant differences in age,noise-exposured working years,intensity of noise exposure,CNE,gender,drinking,hypertension prevalence and noise exposure level in the hearing loss group(P>0.05),while there were statistically difference in s

关 键 词：听力损失 噪声 谷胱甘肽还原酶基因 易感性 巢式病例对照研究 单核苷酸多态性 

分 类 号：R76[医药卫生—耳鼻咽喉科]