L-瓜氨酸对游泳力竭小鼠生长性能及血清生化指标影响的研究  

Study of L-citrulline on Growth Performance of Swimming Exhausted and Serum Biochemical Effect of Mice

作　　者：赵国栋[1] 李晓斌[1] 李婷婷[1] 陈晖[1] 马晨[1] 曾无霜 臧长江[1] ZHAO Guodong;LI Xiaobin;LI Tingting;CHEN hui;MA Chen;ZENG Wushuang;ZANG Changjiang(Laboratory of Animal Genetic Breeding&Reproduction,Xinjiang Agricultural University,Urumqi 830052,China)

机构地区：[1]新疆农业大学动物遗传育种与繁殖实验室,乌鲁木齐830052

出　　处：《中国畜牧兽医》2020年第4期1035-1040,共6页China Animal Husbandry & Veterinary Medicine

基　　金：新疆农业大学研究生科研创新项目(XJAUGRI2019006);大学生创新项目。

摘　　要：试验通过在饮水中添加不同剂量的L-瓜氨酸(L-Cit),探究其对游泳力竭强应激后,小鼠的生长性能及血清生化指标的影响。选用36只平均体重为(14.58±0.77)g的断奶雌性昆明小鼠,随机分为3组,每组6个重复。对照组饮用自来水,试验Ⅰ组和Ⅱ组在饮水中分别添加0.5%和1.0%的L-Cit。试验期21 d,每3 d空腹称重后在(31±2)℃水桶中负重游泳至力竭,于试验第21天摘眼球采血。结果表明,与对照组相比,试验Ⅰ组、Ⅱ组在第6、9、12天时,雌鼠体重分别提高了12.88%(P<0.05)、20.30%(P<0.01)、7.99%(P<0.05)和21.27%(P<0.01)、24.47%(P<0.01)、19.46%(P<0.01)。第15天时试验Ⅱ组小鼠体重比对照组提高13.71%(P<0.01)。与对照组相比,试验Ⅰ组小鼠血清中抗超氧阴离子自由基(ASAFR)活力提高了31.49%(P<0.01);试验Ⅱ组血清中谷胱甘肽过氧化物酶(GSH-Px)活性和抑制羟自由基(IHFR)能力分别提高54.35%(P<0.01)和13.10%(P<0.01);与对照组和试验Ⅰ组相比,试验Ⅱ组小鼠血清白蛋白(ALB)水平分别提高13.16%(P<0.01)和6.28%(P<0.01)。综合试验结果,饮水中添加L-Cit可提高运动后小鼠的生长性能、抗氧化性能及血清总蛋白(TP)和白蛋白含量。综合比较,添加1.0%L-Cit(69.09 mg/d)效果更好。Effects of adding different doses of L-citrulline(L-Cit)in drinking water on the growth performance and serum biochemical indexes of swimming exhausted Kunming mice was studied in this paper.36 weaned females with average weight of(14.58±0.77)g were selected and randomly divided into 3 groups with 6 replicates in each group.The mice in control group drank from tap water,and 0.5%and 1%of L-Cit were added in the drinking water for test groupⅠandⅡ,respectively.The trial lasted 21 d.Every 3 d,the mice were weighed in an empty stomach and then swam with weight loading in(31±2)℃bucket until exhausted.Blood was collected from the eyeballs at day 21.The results showed that:Compared with control group,at the day 6,9 and 12,the body weights of the female mice in test groupsⅠandⅡincreased by 12.88%(P<0.05),20.30%(P<0.01),7.99%(P<0.05)and 21.27%(P<0.01),24.47%(P<0.01),19.46%(P<0.01)respectively.And it increased by 13.71%(P<0.01)at day 15 in test groupⅡ.The serum activity of anti superoxide anion free radical(ASAFR)increased by 31.49%(P<0.01)in test groupⅠ.The serum activity of GSH-Px and inhibit hydroxyl free radical(IHFR)ability were increased by 54.35%(P<0.01)and 13.10%(P<0.01)in test groupⅡ,respectively.Compared with the control group and test groupⅠ,the serum levels of serum albumin(ALB)content were respectively increased by 13.16%(P<0.01)and 6.28%(P<0.01).In conclusion,Adding L-Cit in drinking water could improve the growth performance and antioxidant capacity,increase serum total protein(TP)and albumin contents in mice after exercise,and adding 0.1%L-Cit(69.09 mg/d)was better.

关 键 词：L-瓜氨酸 小鼠 生长性能 抗氧化指标 

分 类 号：S816.7[农业科学—饲料科学]