三个油菜F1组合小孢子培养及双单倍体结实性分析  

Microspore culture and double haploid seed setting analysis in three rapeseed F1 hybrids

作　　者：万丽丽 王转茸 杨光圣[2] 辛强[2] 洪登峰[2] 孙玉宏 高红霞 WAN Li-li;WANG Zhuan-rong;YANG Guang-sheng;XIN Qiang;HONG Deng-feng;SUN Yu-hong;GAO Hong-xia(Institute of Crop,Wuhan Academy of Agricultural Sciences,Wuhan 430065,China;National Key Laboratory of Crop Genetic Improvement,Huazhong Agricultural University,Wuhan 430070,China)

机构地区：[1]武汉市农业科学院作物研究所,武汉430065 [2]华中农业大学作物遗传改良国家重点实验室,武汉430070

出　　处：《湖北农业科学》2020年第1期32-38,77,共8页Hubei Agricultural Sciences

基　　金：中国博士后基金项目(2019M652726);武汉市农业科学院2019年开放课题(KFKT201914);武汉市农业科学院2019年人才项目(KFKT201914)。

摘　　要：为了研究不同油菜(Brassica napus L.)基因型小孢子培养试验中不同浓度秋水仙碱和氟乐林加倍剂对不同基因型材料小孢子再生、加倍率以及成株结实性的影响,选取3个遗传背景不同的F1杂交组合,利用秋水仙碱或氟乐林处理小孢子细胞以及对从小孢子直接再生的植株根系进行秋水仙碱浸泡处理,采用流式细胞仪(FCM)检测不同处理方式下小孢子成苗的倍性水平,并对双单倍体植株开展结实性考察分析。结果表明,3个F1组合的小孢子细胞经过85 mg/L秋水仙碱处理后得到的再生苗数目最多,并且所得到的双单倍体植株的数目最多。不同浓度的氟乐林加倍剂处理试验显示,10μmol/L氟乐林处理小孢子后获得双单倍体相比其他浓度氟乐林处理后的数目多。3个F1组合的小孢子胚在4℃处理10 d后能够显著提高胚的一次成苗率。对3个F1组合的小孢子自然加倍和加倍剂处理后获得的DH群体的结实性考察结果显示,自然加倍获得的双单倍体植株的平均角果长、平均每角果粒数和平均千粒重与不同浓度秋水仙碱或者氟乐林处理后获得的双单倍体无显著差异。To investigate the ploidy levels of microspore-derived plants and effect of colchicine and trifluralin treatment on regeneration,double haploid,and seed setting of regenerated plants in oilseed rape(Brassica napus L.),the effects of microspore culture treatment with antimitotic agents colchicine and trifluralin on the frequency of embryo formation,embryo development,plant regeneration and diploidization rate in three F1 hybrids of rapeseed cultivars were compared,and the ploidy levels of young plantlets were measured by flow-cytometry(FCM).The results indicated three F1 combinations obtained the most number of regenerated plantlets after treatment with 85 mg/L colchicine.The mean rates of DH plants from colchicine(85 mg/L)were higher than the other colchicine concentration,and maximum DH plants were derived from the trifluralin treatment(10μmol/L).After 4℃cold treatment with embryos derived from three F1 hybrid combination for 10 days,the number of embryos to plants conversion(EPC)was significantly increased.The fruity characters of DH population obtained by natural doubling and doubling agent of three F1 combinations were investigated,and the results showed that the average pod length,pod number and 1000-grain weight of double haploid plants treated from antimitotic agents was similar to the spontaneous double haploid plants.

关 键 词：油菜(Brassica NAPUS L.) 小孢子培养 秋水仙碱 氟乐林 倍性分析 结实性 

分 类 号：S565.4[农业科学—作物学]