CDC27基因在系统性红斑狼疮中的表达和临床意义  

Expression and significance of cell division cycle protein 27 gene on peripheral blood mononuclear cell from patients with systemic lupus erythematosus

作　　者：罗清[1] 张露 黄自坤[1] 李俊明[1] Luo Qing;Zhang Lu;Huang Zikun(Dept of Clinical Laboratory, The First Affiliated Hospital of Nanchang University, Nanchang 330006)

机构地区：[1]南昌大学第一附属医院检验科,南昌330006

出　　处：《安徽医科大学学报》2020年第2期292-295,共4页Acta Universitatis Medicinalis Anhui

基　　金：江西省重点研发计划项目(编号:20181BBG70013);江西省教育厅科学技术研究项目(编号:170008);江西省自然科学基金(编号:20171BAB205113);国家自然科学基金项目(编号:81360459)。

摘　　要：目的探讨细胞分化周期蛋白27(CDC27)基因在系统性红斑狼疮(SLE)患者外周血单个核细胞上的表达及临床意义。方法应用反转录酶-聚合酶链反应(RT-PCR)检测32例SLE患者和21例对照者外周血单个核细胞上cdc27的表达,比较两组之间cdc27的表达水平,分析其与SLE临床和治疗的关系,且采用受试者工作特征(ROC)曲线分析其诊断效能。结果SLE组患者外周血单个核细胞的cdc27水平显著低于对照组,差异有统计学意义(Z=126.0,P<0.001),且与C-反应蛋白(CRP)(rs=-0.462,P=0.009),血沉(ESR)(rs=-0.358,P=0.048)、单核细胞数量(rs=-0.386,P=0.029)呈负相关。治疗后SLE患者外周血单个核细胞cdc27表达水平上调(P=0.005)。SLE组患者和对照组ROC曲线下面积(AUC)为0.813(95%CI:0.681~0.944;P<0.001),敏感性为81.25%,特异性为80.95%。结论SLE患者外周血单个核细胞上cdc27的表达水平下调,且与SLE患者的炎症水平以及治疗密切相关,可能作为SLE的潜在诊断指标之一。Objective To investigate the expression of cell division cycle protein 27 gene(cdc27)on peripheral blood mononuclear cell(PBMC)from systemic lupus erythematosus(SLE)patients and its significance.Methods The expression of cdc27 on PBMC from 32 SLE patients and 21 healthy controls(HC)was examined by reverse transcription-polymerase chain reaction(RT-PCR).The expression of cdc27 on PBMC was compared between SLE patients and HC.Correlations of cdc27 on PBMC with several clinical indexes were analyzed.We also analyzed regular treatment influence on expression of cdc27.Moreover,the receiver operating characteristic(ROC)was used to analyze the diagnostic value of cdc27.Results The expression of cdc27 on PBMC in SLE patients was significantly lower than that in HC(U=126.0,P<0.001).The expression of cdc27 on PBMC in SLE patients was negatively correlated with CRP(rs=-0.462,P=0.009),ESR(rs=-0.358,P=0.048),monocytes numbers(rs=-0.386,P=0.029).The expression of cdc27 on PBMC in SLE patients was significantly increased after effective treatment(P=0.005).The area under ROC curve(AUC),sensibility and specificity of cdc27 on PBMC between SLE patients and HC were 0.813(95%CI:0.681~0.944;P<0.001),81.25%,80.95%.Conclusion The expression of cdc27 on PBMC is decreased in SLE patients and correlated with inflammation and treatment.This study suggests that the cdc27 on PBMC may be a potential diagnosis biomarker of SLE patients.

关 键 词：系统性红斑狼疮 细胞分化周期蛋白27基因 诊断标志物 

分 类 号：R593.241[医药卫生—内科学]