基于网络药理学平台的蒙药额力根-7抗肝纤维化作用的机制研究  

Study on the mechanism of action of Eligen-7 on liver fibrosis based on network pharmacology platform

作　　者：谭真真 马月宏 马丽杰 TAN Zhen-zhen;MA Yue-hong;MA Li-jie(Department of Pharmacology,Basic Medical College,Inner Mongolia Medical University,Hohhot 010110,China)

机构地区：[1]内蒙古医科大学基础医学院药理学教研室,内蒙古呼和浩特010110

出　　处：《中国民族医药杂志》2020年第3期36-39,共4页Journal of Medicine and Pharmacy of Chinese Minorities

基　　金：国家自然科学基金资助项目(基于基因表达谱分析蒙古山萝卜花单、复方抗大鼠肝纤维化作用研究NO.81560706);内蒙古自然科学基金资助项目(蒙药七味红花散对大鼠肝纤维化模型的治疗作用及蛋白组学研究No.2014MS0841);国家自然科学基金资助项目(蒙药古日古木单、复方对肝损伤的保护作用及JNK通路机制研究N0.81360676)。

摘　　要：目的:探讨额力根-7抗肝纤维化的作用机制.方法:通过网络药理学平台(TCMSP)对额力根-7的化学成分及作用靶点进行检索,并通过Uniprot、Genecards数据库确定基因,采用Cytoscape3.7.1软件构建成分-靶点网络.通过OMIM数据库查询与肝纤维化相关的靶点,通过蛋白质相互作用(PPI),获得核心靶点,通过生物信息注释数据库(DAVID)进行基因本体(GO)功能富集和基于京都基因与基因组百科全书(KEGG)通路富集分析.结果:PPI核心网络有19个节点,关键蛋白涉及IGF1、EGFR、TNF、TGFB1等.GO功能富集分析按P<0.05筛选到GO条目110个,KEGG通路富集分析按P<0.05筛选到通路30条.结论:初步探索了额力根-7抗肝纤维化可能的作用机制,为额力根-7进一步的研究提供参考.Objective:To investigate the mechanism of Eligen-7 anti-liver fibrosis.Methods:The chemical constituents and targets of Eligen-7 were searched by the analysis of traditional Chinese medicine system pharmacology platform(TCMSP),and the target-related genes was determined from Uniprot and Genecards databases,the compound-targets networks was constructed by Cytoscape 3.7.1.The core target is obtained through the protein-protein interaction(PPI)network.Finally,gene ontology(GO)functional enrichment analysis and pathway enrichment analysis based on Kyoto Encyclopedia of Genes and Genomes(KEGG)were performed through DAVID.Results:The PPI core network has 19 nodes,and the key targets involved IGF1,EGFR,TNF,TGFB1,etc.The functional enrichment analysis of GO screened 110 items according to P<0.05,the pathway enrichment analysis screened 30 signaling pathway.Conclusion:This study initially explored the active ingredients and possible mechanism of Eligen-7 in the treatment of liver fibrosis,to provide reference for further research of Eligen-7.

关 键 词：网络药理学 七味清肝散 肝纤维化 靶点 信号通路 

分 类 号：R291.2[医药卫生—民族医学]