12C^6+离子束诱变选育温度耐受螺旋藻高产藻株及其培养条件的优化  

Screening temperature-tolerant and high-yield Spirulina maxima mutants using 12C^6+ion beam irradiation and optimizing its culture conditions

作　　者：王丽娟[1,3] 郑天翔 杨宋琪 李欣 罗光宏[1,3] WANG Lijuan;ZHENG Tianxiang;YANG Songqi;LI Xin;LUO Guanghong(Gansu Tech Innovation Center of Microalgae,Hexi University,Zhangye 734000,China;College of Agriculture and Ecological Engineering,Hexi University,Zhangye 734000,China;Key Laboratory of Hexi Corridor Resources Utilization of Gansu,Zhangye 734000,China;Institute of Modern Physics,Chinese Academy of Sciences,Lanzhou 730000,China)

机构地区：[1]河西学院甘肃省微藻技术创新中心,张掖734000 [2]河西学院农业与生态工程学院,张掖734000 [3]甘肃省河西走廊特色资源利用重点实验室,张掖734000 [4]中国科学院近代物理研究所,兰州730000

出　　处：《辐射研究与辐射工艺学报》2020年第2期25-32,共8页Journal of Radiation Research and Radiation Processing

基　　金：国家自然科学基金项目(11665011);甘肃省高校协同创新科技团队支持计划项目(2017C-17);甘肃省高等学校科研项目(2017A-086);甘肃省重点研发计划项目(18YF1WG087)资助。

摘　　要：以极大螺旋藻(Spirulina maxima)为实验材料,利用不同剂量的12C^6+离子束对其进行辐照诱变,采用平板分离和温度选育初筛变异藻株,通过生物量和遗传稳定性复筛温度耐受高产突变株,并优化其培养条件。结果表明,在吸收剂量为600 Gy和800 Gy时对藻株的诱变效果显著,致死率为71.84%和78.47%,筛选出的2株温度耐受突变株,分别是经600 Gy辐照后20℃培养和经800 Gy辐照后35℃培养条件下的螺旋藻突变株Sm04#和Sm21#。经过10次传代培养,生物量分别达到0.54 g/L和0.91 g/L,较对照组提高了11.49%和12.64%,且遗传稳定性良好。培养条件的优化实验表明,Sm04#和Sm21#的最适pH均为9.0,最适光强分别为70μmol/(m2·s)和80μmol/(m2·s),最适光暗周期依次为12∶12 h和14∶10 h。进一步对2株突变株在室内扩大培养,蛋白质和多糖含量均高于对照组。研究结果为12C^6+离子束辐照诱变螺旋藻藻株的开发和应用提供理论依据。Spirulina maxima(S.maxima)was irradiated using 12C^6+ion beams at different doses,and the mutant strains were selected by plate separation and temperature treatment methods.Mutant strains with high biomass yields and increased temperature tolerance were screened out by genetic stability analysis.The culture conditions of the mutants were also optimized.Results showed that when the absorbed doses were 600 Gy and 800 Gy,the mutagenic effects on the algae strains were significant and the mortality rates were 71.84%and 78.47%,respectively.Two temperature-tolerant mutants(Sm04#and Sm21#)were obtained,which were cultured at 20℃and 35℃after irradiation at 600 Gy and 800 Gy.After ten generations,the biomasses of the mutants were 0.54 g/L and 0.91 g/L,respectively,which were 11.49%and 12.64%larger than the biomass of the control group,respectively.The culture conditions optimization experiment indicated that the optimum pH of both Sm04#and Sm21#was 9.0,the optimum light intensities were 70 and 80μmol/(m2·s),respectively,and the optimum light-dark periods were 12:12 h and 14:10 h,respectively.In addition,the protein and polysaccharide contents of the two mutants were both higher than those of the control group,which provides a theoretical basis for the development and application of S.maxima using carbon ion irradiation.

关 键 词：极大螺旋藻 12C^6+离子束 温度耐受 培养条件优化 

分 类 号：Q815[生物学—生物工程] TL99[核科学技术—核技术及应用]