MicroRNA-155/FOXO3a在心脏手术后心房颤动患者心房组织中的表达及与心房纤维化的关系  

Expression of miR-155/FOXO3a axis in atrial tissue of patients with atrial fibrillation after cardiac surgery and its relationship with atrial fibrosis

作　　者：冶敦清 祁国荣 路霖 王黎明 Dun-qing Ye;Guo-rong Qi;Lin Lu;Li-ming Wang(Department of Cardiac Surgery,Qinghai Cardiovascular and Cerebrovascular Disease Hospital,Xining,Qinghai 810000,China)

机构地区：[1]青海省心脑血管病专科医院心外科,青海西宁810000

出　　处：《中国现代医学杂志》2020年第8期40-44,共5页China Journal of Modern Medicine

摘　　要：目的探讨microRNA-155/叉头框蛋白O 3a(miR-155s/FOXO3a)在心脏手术后心房颤动(以下简称房颤)患者心房组织中的表达情况,及其与心房纤维化的关系。方法选取2018年2月—2019年4月青海省心脑血管病专科医院收治的风湿性心脏瓣膜病并行心脏手术治疗的患者92例。根据患者病史、心电图及24 h动态心电图检查结果分为房颤组和窦性心律组。于心脏手术中采集患者右心房组织,采用qRTPCR检测心房组织中miR-155、FOXO3a mRNA相对表达量;采用免疫印迹法检测心房组织中FOXO3a和p-FOXO3a蛋白相对表达量;采用Masson染色法及计算机成像分析系统计算心房组织胶原容积分数,评价心房组织纤维化程度;采用Pearson法分析心房组织miR-155、p-FOXO3a蛋白水平与心房纤维化程度的相关性。结果房颤组左心房内径、右心房内径高于窦性心律组(P<0.05)。房颤组胶原容积分数较窦性心律组高(P<0.05)。窦性心律组miR-155 mRNA相对表达量低于房颤组(P<0.05),FOXO3a mRNA相对表达量高于房颤组(P<0.05)。房颤组p-FOXO3a蛋白相对表达量低于窦性心律组(P<0.05)。房颤组患者心房组织miR-155水平与p-FOXO3a蛋白水平呈负相关(r=-0.485,P<0.05),与胶原容积分数呈正相关(r=0.490,P<0.05),心房组织p-FOXO3a蛋白水平与胶原容积分数呈负相关(r=-0.471,P<0.05)。结论心脏手术后房颤患者心房组织中miR-155高表达,p-FOXO3a蛋白低表达,均与心房纤维化有关,共同影响房颤的发生与维持。Objective To investigate the expression of microRNA-155/fork-head box protein O 3a(microRNA-155/FOXO 3a)axis in atrial tissue of patients with atrial fibrillation after cardiac surgery and its relationship with atrial fibrosis.Methods Ninety two patients with rheumatic valvular heart disease who underwent cardiac surgery in Qinghai Provincial Cardiovascular and Cerebrovascular Disease Hospital from February 2018 to April 2019 were selected and divided into atrial fibrillation group(43 cases)and sinus rhythm group(49 cases)according to their medical history,electrocardiogram(ECG)and 24-hour dynamic electrocardiogram(DCG).Right atrial tissue was collected during cardiac surgery,real-time quantitative polymerase chain reaction(RT-qPCR)was used to detect the expressions of microRNA-155 and FOXO3a in atrial tissue,the expression levels of FOXO3a and p-FOXO3a in atrial tissue were detected by immunoblotting(WB),the collagen volume fraction(CVF)of atrial tissue was calculated by Masson staining and computer imaging analysis system to evaluate the degree of atrial fibrosis,and Pearson method was used to analyze the correlations of microRNA-155,p-FOXO3a protein levels and CVF in atrial tissue.Results Compared with sinus rhythm group,the diameters of left atrium and right atrium increased significantly,the level of microRNA-155 and CVF in atrial tissue increased significantly,and the levels of FOXO3a mRNA and p-FOXO3a protein decreased significantly(all P<0.05).The levels of microRNA-155 and p-FOXO3a protein in atrial tissue were negatively correlated(r=-0.485,P<0.05),while the levels of microRNA-155 in atrial tissue were positively correlated with CVF(r=0.490,P<0.05),and the level of p-FOXO3a protein in atrial tissue was negatively correlated with CVF(r=-0.471,P<0.05).Conclusions The high expression of microRNA-155 and low expression of p-FOXO3a protein in atrial tissue of patients with atrial fibrillation after cardiac surgery are both related to atrial fibrosis,which affect the occurrence and maintenance of atrial fibri

关 键 词：心房颤动 MIR-155 叉头框蛋白O 3a 心房 组织 纤维化 

分 类 号：R541.75[医药卫生—心血管疾病]