摇瓶法培养甲烷氧化菌吸附甲烷氧化菌素的研究  

Adsorption of methanobactin released by methanotrophs from flask fermentation

作　　者：张帅[1] 郝雪 王悦[1] 辛嘉英[1] ZHANG Shuai;HAO Xue;WANG Yue;XIN Jia-ying(Key Laboratory for Food Science and Engineering,Harbin University of Commerce,Harbin 150076,China)

机构地区：[1]哈尔滨商业大学黑龙江省普通高等学校食品科学与工程重点实验室,哈尔滨150076

出　　处：《哈尔滨商业大学学报（自然科学版）》2020年第2期210-213,共4页Journal of Harbin University of Commerce：Natural Sciences Edition

基　　金：国家自然科学基金项目(21573055);哈尔滨商业大学校级科研项目(18XN043);哈尔滨商业大学研究生创新科研项目(YJSCX2018-540HSD)。

摘　　要：甲烷氧化菌素(methanobactin,mb)是甲烷氧化菌分泌的一种可结合铜的小分子荧光肽,具有氧化还原活性.采用摇瓶法培养甲烷氧化菌,通过不同培养基装液量来寻找菌体的生长规律,确定最适生长条件,并采用大孔树脂法分离纯化甲烷氧化菌素,通过大孔树脂对甲烷氧化菌素的静态吸附及动态吸附方式对比,找到最佳的吸附效果.实验结果表明,摇瓶法培养的最佳培养基装液量1.5 L,通过大孔树脂静态吸附是最佳的吸附甲烷氧化菌素方式,为今后提高甲烷氧化菌素的分离能力奠定了科学基础.Methanobactin(mb)was a small molecule fluorescent peptide that bond to copper from methanotrophs,and had redox activity.After methanotrophs were cultured by shake-flask fermentation,andthe optimum growth conditions were determinated by different volume of medium.According to the difference between static adsorption and dynamic adsorption of macroporous resin,the better adsorption effect was found.The results showed that the optimum volume of culture medium was 1.5 L,and the static adsorption of macroporous resin was the better way to obtainmethanobactin than dynamic adsorption.The results had proved useful information ofimproving methanobactin productivity.

关 键 词：甲烷氧化菌 甲烷氧化菌素 大孔树脂 静态吸附 动态吸附 

分 类 号：Q93-33[生物学—微生物学]