姬松茸液体发酵培养基筛选和条件优化研究  

Study on Screening and Condition Optimization of Liquid Fermentation Medium for Agaricus blazei Murill

作　　者：侯宪文[1] 符瑞益[1] 徐林[1] 文少白[2] HOU Xianwen;FU Ruiyi;XU Lin;WEN Shaobai(Environment and Plant Protection Institute,CATAS/Hainan Key Laboratory of Tropical Eco-Circular Agriculture,Haikou 571101,China;School of Tropical and Laboratory Medicine,Hainan Medical University,Haikou 571199,China)

机构地区：[1]中国热带农业科学院环境与植物保护研究所/海南省热带生态循环农业重点实验室,海口海南571101 [2]海南医学院热带医学与检验医学院,海口海南571199

出　　处：《广东农业科学》2020年第2期24-29,共6页Guangdong Agricultural Sciences

基　　金：农业农村部农业技术试验示范与服务支撑项目(SMH2019-2021);中国热带农业科学院基本科研业务费专项资金(2016hzs1J010)。

摘　　要：【目的】研究姬松茸液体发酵培养基最佳组合配方和发酵条件。【方法】以姬松茸菌丝量为评价指标,采用单因子和正交实验设计相结合的方法优化姬松茸液体发酵培养基配方。【结果】单因子实验条件下最适发酵条件为:以甘露醇为碳源、硫酸铵为氮源、磷酸二氢钠为其他营养元素,pH值为5.0。而通过正交设计不同组合培养,确定姬松茸液体发酵培养基的最佳配方为:以葡萄糖为碳源、硫酸铵为氮源、磷酸二氢钠为其他营养元素,液体初始pH值为6.0。在25℃下150 r/min振荡培养5 d,100 mL发酵液中姬松茸菌丝干重达到0.4463±0.0132 g。【结论】单因子培养和正交组合培养结果存在一定差异,在进行菌株发酵培养时,需将两种方法结合才能更准确地确定其培养和发酵条件。姬松茸液体发酵能快速实现菌株的大量扩培,为姬松茸进一步栽培或提取发酵产物奠定基础。【Objective】The study was conducted to explore the optimum formula and fermentation condition of liquid fermentation medium for Agaricus blazei Murill.【Methods】With the dry weight of A.blazei mycelium as the index,the liquid fermentation medium formula was optimized by single factor experiments and orthogonal experiments.【Results】Under the conditions of single factor experiment,the optimal fermentation conditions were taking mannitol as carbon source,(NH4)2SO4 as nitrogen source,NaH2PO4 as other nutrient element with pH of 5.0.Through orthogonal design of different culture combinations,the best formula of A.blazei liquid fermentation medium were taking glucose as carbon source,(NH4)2SO4 as nitrogen source and NaH2PO4 as other nutrient element with the initial pH of 6.After conducting 150 r/min shaking culture at 25 ℃ for 5 days,the dry weight of A.blazei mycelium in 100 mL fermentation broth reached 0.4463±0.0132 g.【Conclusion】The results showed that there was a certain difference between single factor culture and orthogonal combination culture.In order to determine the culture and fermentation conditions more accurately,it is needed to combine the two methods when conducting fermentation culture.In addition,the liquid fermentation of A.blazei can rapidly achieve a large number of strain expansion,which lays a foundation for further cultivation or extraction of fermentation products.

关 键 词：食药兼用真菌 姬松茸 培养条件 菌丝干重 正交设计 

分 类 号：S646.9[农业科学—蔬菜学]