舟山群岛地区蝙蝠博卡病毒的初步鉴定与分析  

PRELIMINARY IDENTIFICATION AND GENETIC ANALYSIS OF BAT BOCAVIRUS IN ZHOUSHAN ISLANDS

作　　者：朱长强 沈柏宇 王依 吕瑞辰 杨露 艾乐乐 胡丹 吕恒 王长军 谭伟龙 ZHU Chang-Qiang;SHEN Bo-Yu;WANG Yi;LYU Rui-Chen;YANG Lu;AI Le-Le;HU Dan;LYU Heng;WANG Chang-Jun;TAN Wei-Long(Centre for Diseases Prevention and Control of Easten Theater,Nanjing,Jiangsu 210002,China;Support Battalion of PLA 31610,Zhoushan,Zhejiang 316000,China;Jiangsu Institute of Parasitosis Diseases,Wuxi 214064,Jiangsu,China;The PLA Center for Disease Control and Prevention,Beijing 100079,China)

机构地区：[1]东部战区疾病预防控制中心,江苏南京210002 [2]解放军31610部队支援保障营,浙江舟山316000 [3]江苏省血吸虫病防治研究所,江苏无锡214064 [4]中国人民解放军疾病预防控制中心,北京100071

出　　处：《寄生虫与医学昆虫学报》2019年第4期214-218,共5页Acta Parasitologica et Medica Entomologica Sinica

基　　金：江苏省“333”工程科研项目(BRA2018410);国家自然科学基金项目(U160223);国家科技重大专项(2017ZX10303401007)。

摘　　要：为进一步了解我国蝙蝠携带博卡病毒(Bocavirus)的分布状况和遗传多样性特征,本研究采集舟山群岛地区4种蝙蝠的肠组织样本,使用套式PCR方法进行蝙蝠博卡病毒检测,分析该地区蝙蝠携带博卡病毒的遗传多样性。结果显示,从141只蝙蝠(菲菊头蝠、马铁菊头蝠、大卫鼠耳蝠和渡濑式鼠耳蝠)体内共检测出7份博卡病毒的DNA目的条带,经测序后获得5条博卡病毒序列,系统进化分析表明蝙蝠源博卡病毒同已发现的其他博卡病毒属于不同的进化分支。序列遗传进化分析显示,5条序列的同源性介于98.9%~100%之间,与其他博卡病毒的同源性介于50.8%~77.2%之间;根据ICTV关于博卡病毒属新种的判定标准,该地区检测出蝙蝠源博卡病毒为新的博卡病毒种类。To investigate the distribution and genetic diversity of bocavirus carried by wild bats in China,the intestinal tissue samples of 4 species of bats were collected from Zhoushan islands,China.Nested PCR was used to detect the potential bocavirus,and the genetic diversity of the bocavirus in these bats was also analyzed based on the partial sequences of VP1 gene.A total of 141 bats was sampled,involved with 4 species Rhinolophus pusillus,R.ferrumequinum,Myotis davidii and My.formosus.Among them,7 objective strips of bocaviruses were detected positive from R.pusillus(n=3)and My.davidii(n=2)respectively.Phylogenetic analysis based on the 5 sequences of the bat bocaviruses belonged to a distinguished evolutionary branch different with other known bocaviruses.The 5 sequences were clustered together with high homologies ranged from 98.9%to 100%,but their nucleotide sequences were shown distinctive differences with other bocavirus in the identities ranged from 50.8%to 77.2%.According to ICTV criteria,the bat-derived bocavirus detected in this area might be a new species of bocavirus.

关 键 词：舟山群岛地区 蝙蝠 博卡病毒 进化分析 

分 类 号：R373[医药卫生—病原生物学]