草莓4种主要病毒检测及SVBV贵州分离物基因组测定及分析  

Detection of Four Strawberry Viruses and Identification and Analysis of Genome of Strawberry Vein Banding Virus Guizhou Isolate

作　　者：王廿[1] 贾蒙骜 黄刚 卯婷婷[1] 赵玳琳 陶刚[1] WANG Nian;JIA Meng-ao;HUANG Gang;MAO Ting-ting;ZHAO Dai-lin;TAO Gang(Institute of Plant Protection,Guizhou Academy of Agricultural Sciences,Guizhou Guiyang 550003,China;Guizhou Academy of Tobacco Science,Guizhou Guiyang 550081,China;Qiandongnan Vocational&Technical College for Nationalities,Guizhou Kaili 556000,China)

机构地区：[1]贵州省农业科学院植物保护研究所,贵州贵阳550003 [2]贵州省烟草科学院,贵州贵阳550005 [3]黔东南州民族职业技术学院,贵州凯里556000

出　　处：《西南农业学报》2020年第3期613-618,共6页Southwest China Journal of Agricultural Sciences

摘　　要：【目的】为了解贵州地区草莓(Fragaria×ananassa)栽培品种中4种主要病毒的发病率和草莓褪绿斑驳病毒(Strawberry vein banding virus,SVBV)贵州分离物与其他分离物的遗传进化关系,对贵州省贵阳市和黔东南州凯里市大棚草莓植株和草莓组培苗进行4种主要草莓病毒检测。【方法】克隆SVBV的全长基因组序列及CP编码的核酸序列后测序并分析。【结果】采集的77份草莓样品检测出SVBV和草莓轻型黄边病毒(Strawberry mild yellow edge virus,SMYEV)2种病毒,未检测到草莓斑驳病毒(Strawberry mottle virus,SMoV)和草莓蚀刻病毒(Strawberry crinkle virus,SCV),其中SVBV的检出率为19.5%,SMYEV为2.6%;SVBV-GZ(GenBank登录号:MH894295)全基因组序列为7859 bp,基因组结构推测有7个开放读框构成,基于SVBV基因组核酸序列的系统发育分析表明SVBV-GZ与SVBV-BJ(GenBank登录号:KR080547)亲缘性较近。【结论】基于SVBV CP氨基酸序列的系统发育分析发现,贵州不同地区草莓SVBV不同分离物与其他中国不同地区分离物同源性较高,可能是由于贵州从全国范围调运草莓种苗造成。【Objective】This paper aims to investigate incidence of four strawberry viruses in Guizhou Province and the evolutionary relationship between strawberry vein banding virus SVBV-GZ and other SVBV isolates.【Method】77 strawberry plants and the tissue culture seedlings were collected from greenhouses located in Guiyang City,Kaili City and Qiandongnan Vocational&Technical College for Nationalities.Strawberry plants were collected for detection of four strawberry viruses.The full genome sequences and CP nucleotide sequences of SVBV were cloned to the vector for sequencing and analysis.【Result】Both SVBV and strawberry mild yellow edge virus(SMYEV)were detected by RT-PCR,and the rate of virus carrying SVBV and SMYEV were 19.5%and 2.6%,respectively.However strawberry mottle virus(SMoV)and strawberry crinkle virus(SCV)viruses were not detected among these samples.The complete genome sequence of SVBV-GZ(GenBank accession No.:MH894295)is 7859 bp,and the genome structure might be composed of seven open reading frames.Phylogenetic analysis showed that the highest homology was observed between SVBV-GZ and SVBV-BJ(GenBank accession No.:KR080547)nucleotide sequences.【Conclusion】The nuclear sequence analysis of SVBV CP showed that the SVBV isolates in Guizhou are the most related to different isolates from different area in China.This may be caused by importing the strawberry seedlings from different area.

关 键 词：草莓 病毒发生率 核酸序列分析 

分 类 号：S66[农业科学—果树学]