测定生物体pH值的磁共振研究进展  

NMR study progress of the pH determination in biological samples

作　　者：魏瑶 邓鹏翅[1] WEI Yao;DENG Peng-chi(Analytical&Testing Center,Sichuan University,Chengdu 610064,China)

机构地区：[1]四川大学分析测试中心,四川成都610064

出　　处：《化学研究与应用》2020年第4期489-500,共12页Chemical Research and Application

基　　金：四川大学实验技术立项项目(20170208)资助。

摘　　要：生物样品的pH值与许多重要的生理活动密切相关。在微观尺度上,局部pH会影响细胞、细胞器和酶的重要生理活动。本文综述了当前对生物样品pH进行检测的一些方法,包括微电极法、荧光分析法和核磁共振法的优点、局限性和应用。并对磁共振光谱法和化学交换饱和转移成像法进行了详细介绍。The pH of biological samples was closely related to many important physiological activities.At the microscopic scale,local pH drastically affected the vital activities of cell,cellular organelles and enzymes.Current methods in biological ph detection,including the advantages of microelectrode method,fluorescence analysis and nuclear magnetic resonance,as well as their limitations and applications are discussed.The magnetic resonance spectroscopy and chemical exchange saturation transfer imaging methods are described in detail.

关 键 词：生物样品 PH 磁共振光谱 动态核极化 化学交换饱和转移 

分 类 号：O652.2[理学—分析化学]